基质金属蛋白酶MMP-2及其抑制物TIMP-2在宫颈癌中的表达  

The expressions of MMP-2 and their inhibitors TIMP-2 in cervical cancer

作　　者：尹香花 隋玉梅 成艳 苏悦 符丹 黄永生 

机构地区：[1]扬州大学临床医学院妇产科,江苏扬州225001

出　　处：《中国现代医学杂志》2012年第15期15-18,共4页China Journal of Modern Medicine

基　　金：江苏省科技厅自然科学基金(No:BK2008205)

摘　　要：目的探讨基质金属蛋白酶家族MMP-2及其抑制物TIMP-2在宫颈癌组织中的表达与宫颈癌临床病理特征的关系,研究MMP-2为研究对象在肿瘤以及细胞外基质(ECM)中的变化,明确其与调节因子TIMP-2间的相互调节关系。方法通过分子生物学手段检测51例宫颈癌组织中MMP-2及TIMP-2的基因表达情况,并与30例宫颈炎作对照,研究MMP-2、TIMP-2等在宫颈癌中的基因和蛋白水平上的变化,明确其相互调节关系。结果 MMP-2、TIMP-2在正常宫颈上皮组织中均无表达,在宫颈癌组织中的阳性表达率分别为74.5%(38/51)、47.1%(24/51),有显著性差异(P<0.01)。MMP-2、TIMP-2的表达与组织学类型无关,但与临床分期、细胞分化程度、淋巴结转移有关。结论 MMP-2及TIMP-2的改变以及表达与宫颈癌浸润深度及淋巴结转移密切相关,三者表达量的检测可作为早期诊断、监测妇科恶性肿瘤的理想标志物。【Objective】 To discuss the different expressions of matrix metalloproteinases（MMP-2） in cervical cancer cells and extracellular matrix（ECM）,study the correlations between regulatory factors by investigating the expression of MMP-2 and reversion inducing cysteine rich protein with kazal motifsand tissue inhibitors of the metalloproteinase（TIMP-2） in cervical cancer tissue and the relationship between their expressions and cervical cancer’s clinical and pathological features.【Methods】 Detect the expression of MMP-2,and TIMP-2 in 51 cases of cervical cancer,30 normal cervical tissue（chronic cervicitis）,study the relationships between them.【Results】 MMP-2 and TIMP-2 have no expression in normal cervical epithelial tissue,while positive expression rate are 74.5%（38/51） and 47.1%（24/51） in cervical cancer tissue,statistical significance（P 〈0.01）.The expression of MMP-2 and TIMP-2 has no relation with histologic type,but related with the clinical stages,degree of cell differentiation and the lymph node metastasis.【Conclusion】 The expressions of MMP-2,TIMP-2 are closely related to cervical cancer infiltration and lymph node metastasis,the amount of three factors can be used as tests of early diagnosis,monitoring of malignant gynecological tumor.

关 键 词：宫颈癌 基质金属蛋白酶 TIMP-2 

分 类 号：R737.3[医药卫生—肿瘤]