应激诱导木聚糖酶基因在枯草芽孢杆菌中表达  

Expression of Xylanase Gene in Bacillus subtilis Induced by Stresses

作　　者：曹鹏涛[1] 杨丽娜[1] 龚月生[1] 张建[1] 杨明明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2012年第3期55-58,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金2008年资助项目(30871813)

摘　　要：克隆含有信号肽碱性木聚糖酶基因(xynA)片段与依赖转录起始因子σB的启动子PgsiB片段,将其克隆至实验室前期构建的大肠杆菌-枯草芽孢杆菌穿梭载体上,并转化入枯草芽孢杆菌WB700菌株,获得重组菌BXS-W。当细菌生长至对数期中期时,施加不同的应激处理,采用DNS法测定木聚糖酶活性。结果显示,获得了xynA与PgsiB片段;成功构建表达载体pBXS,并获得重组菌株BXS-W;应激试验表明,施加的5种应激条件均极显著提高(P<0.01)木聚糖酶基因的表达量,其中以乙醇应激效果最好。An alkali-tolerant xylanase gene（xynA） with signal peptides gene and promoter PgsiB gene were got by PCR and were cloned into the E.coli-Bacillus subtilis shuttle vector.We transformed this recombinant vector into Bacillus subtilis stain WB700 and got recombinant strain.The recombinant strain accepted different stresses when grown in LB medium to the mid-logarithmic growth phase.Then xylanase activity was assayed by DNS method.Results showed that we constructed recombinant plasmid pBXS and got recombinant strain BXS-W.All five stresses could quite significantly improve the expression of xylanase gene（P〈0.01）.Ethanol stress was better than other four stresses.

关 键 词：木聚糖酶 枯草芽孢杆菌 应激处理 PgsiB启动子 

分 类 号：Q786[生物学—分子生物学]