白腐菌对十溴联苯醚的酶促降解研究  

Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium

作　　者：熊士昌[1] 尹华[1] 何宝燕[1] 彭辉[1] 龙焰[1] 叶锦韶[1] 张娜[1] 彭素芬[1] 

机构地区：[1]暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州510632

出　　处：《环境化学》2012年第5期615-619,共5页Environmental Chemistry

基　　金：国家自然科学基金委-广东省联合基金重点项目(U0933002);国家自然科学基金项目(50978122);中央高校基本科研业务费专项资金资助项目(21611343)资助

摘　　要：考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg.L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30℃、pH 4—5、BDE-209浓度1 mg.L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.Degradation of BDE-209 by the cell,extracellular and intracellular enzymes of P.chrysosporium was investigated,and after 7 days 60.17%,54.14% and 22.32% BDE-209 was degraded respectively at an initial BDE-209 concentration of 1 mg·L-1,which demonstrated that BDE-209 was mainly degraded by extracellular enzymes.The effect of temperature,pH,and BDE-209 concentration on the degradation of BDE-209 was further studied.The results indicated that the optimal conditions of BDE-209 degradation were as follow： temperature 30 ℃,pH 4—5,BDE-209 concentration 1 mg·L-1.Infrared spectroscopy analysis showed that the degradation of BDE-209 was related to Br—C、CH2—O—CH2.The GC-MS chromatogram revealed that deprivation of Br occured during the degradation of BDE-209.

关 键 词：BDE-209 胞外酶 降解 

分 类 号：X172[环境科学与工程—环境科学]