苯对尾悬吊大鼠血细胞及脂质过氧化的影响  

Effects of Benzene Exposure on Blood System and Lipidperoxidation in Rats Weightlessness Simulated by Tail-suspension

作　　者：李曙光[1] 何新星[1] 吴大蔚[1] 刘洪涛[1] 肇海[1] 许峰[1] 高郁晨[1] 彭远开[1] 杨成佳[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《航空航天医学杂志》2012年第6期694-697,共4页Journal of Aerospace medicine

基　　金：载人航天领域预先研究项目(010204)

摘　　要：目的:探讨苯吸入染毒对尾吊模拟失重大鼠血液系统、脂质过氧化水平及抗氧化酶的影响。方法:将20只雄性SD大鼠随机分为2组:对照组和染毒组。对照组吸入新鲜空气,染毒组吸入10 mg/m3的苯气体,每天24 h,连续7 d。结果:与对照组相比,染毒组中白细胞(WBC)显著低于对照组(P<0.05);血红蛋白(HGB)显著低于对照组(P<0.05);淋巴细胞(LYM)极显著低于对照组(P<0.01);嗜碱性粒细胞(BAS)显著高于对照组(P<0.05);T淋巴细胞亚群分类:CD4极显著降低(P<0.01)、CD8极显著升高(P<0.01)。染毒组中血清超氧化物歧化酶SOD活力极显著低于对照组(P<0.01);血清谷胱甘肽过氧化酶GSH-PX活力极显著高于对照组(P<0.01)。结论:苯吸入染毒7 d可导致尾吊SD大鼠血液学指标的改变,表现为WBC降低,HGB降低,淋巴细胞(LYM)降低,BAS升高;HGB明显降低,RDW明显升高。脂质过氧化及抗氧化酶指标的改变,表现为SOD活力降低和GSH-PX活力升高。Objective：To study effects of benzene exposure on blood systemand lipid peroxidation and activity of anti-exidase in rats weightlessness simulated by tail-suspension.Methods：Twenty Male SD rats ware divided randomly into two groups,control group with air and benzene group with breathed at the dose of 10 mg/m3 benzene for 7 days.Results：As compared with control group,white blood cell（WBC） decreased significantly（P0.05）,blood hemoglobin（HGB） decreased significantly（P0.05）,blood lymphocyte（LYM） decreased especial significantly（P0.01）.basophil（BAS） increased significantly（P0.05）,blood T-Lymphocytes subgroup typing：CD4 decreased especial significantly（P0.01）,CD8 increased especial significantly（P0.01）.Activity of superoxide dismutase（SOD） decreased especial significantly（P0.01）,activity of glutathione peroxidase（GSH-PX） increased especial significantly（P0.01）.Conclusions：The above results showed that benzene exposure could cause change in white blood cell differential count,blood lymphocyte（LYM） decreased especial significantly;SOD decreased especial significantly,and compensatively increase activity of GSH-PX.

关 键 词：尾吊 血液 毒性 脂质过氧化 抗氧化酶 

分 类 号：R-332[医药卫生]