^(90)Y标记抗人成骨肉瘤单抗体内生物分布  

Study of Biodistribution of OSMcAb Labeled with ^(90)Y

作　　者：纪方[1] 苗积生 沈茜 史丽梅[4] 

机构地区：[1]第二军医大学长海医院骨科,上海200433 [2]上海第一肺科医院免疫室,200433 [3]上海医院实验诊断科,200433 [4]解放军第208医院检验科,长春136020

出　　处：《中国矫形外科杂志》2000年第3期258-260,共3页Orthopedic Journal of China

摘　　要：目的 :研究抗人成骨肉瘤单抗 (OSMcAb)标记放射性核素90 Y在动物体内生物分布情况。方法 :应用DTDA环酸酐法将90 Y标记抗人成骨肉瘤单克隆抗体 (OSMcAb) ,动物体内观察12 5Ⅰ OSMcAb与90 Y OSMcAb生物分布情况及不同90 Y标记物体内分布情况 ,在裸鼠体内观察90 Y OSMcAb肿瘤内浓度现象。结果 :90 Y标记OSMcAb动物体内生物分布与12 5Ⅰ OSMcAb相似 ,不同络合物配体影响90 Y体内生物分布 ,经90 Y标记OSMcAb在肿瘤内浓聚。结论 :90 Y标记的OSMcAb体内有稳定的分布规律 ,不同标记物配体影响90 Y体内分布。Objective: Study biodistribution of DTPA Coupled OSMcAb labeled with 90 Y. Methods: The OSMcAb was labeled with 90 Y using cyclic DTPA Chelation. Research the biodistribution of 90 Y OSMcAb and 125 Ⅰ OSMcAb. Compare the distribution of different 90 Y Complex in viro. The specific of 90 Y OSMcAb was investrigated with distribution of 90 Y Cl 3/ 90 Y OSMcAb in nude mice. Results: The distribution of 90 Y OSMcAb was similar to 125 Ⅰ OSMcAb in viro. The coupled complex affected the distribution of 90 Y complex in viro. 90 Y rdaioisotopes concentrated in tumor targeted by OSMcAb. Conclusion: 90 Y OSMcAb had a regular distribution in viro. Results seem thi radio immunotherapy feasible.

关 键 词：单克隆抗体 钇放射性同位素 生物分布 骨肉瘤 

分 类 号：R738.1[医药卫生—肿瘤]