MMP-2与TIMP-2对大鼠创面愈合及愈合后瘢痕影响的对比性研究  

Effects of MMP-2 and TIMP-2 to wound healing and healing scar in rats:Comparative Study

作　　者：郭晋宣[1] 杨时昕[2] 赵威[1] 杨丽丽[1] 王海龙[1] 沈欣 

机构地区：[1]山西医科大学 [2]山西医科大学附属第一医院烧伤整形科 [3]山西省朔州市怀仁县93582部队后勤部卫生办

出　　处：《中国医疗前沿》2012年第7期22-23,33,共3页China Healthcare Innovation

摘　　要：目的观察皮肤创伤愈合过程中小剂量医学参考范围之内的外源性基质金属蛋白酶(MMP-2)及基质金属蛋白酶抑制剂(TIMP-2)对创面愈合及愈合后瘢痕的影响。方法选取SD大鼠32只,建立大鼠背部皮肤创伤愈合模型,将其随机分成MMP-2组、TIMP-2组、磷酸盐缓冲液(PBS)对照组,分别给予大鼠创面上述三种试剂,对各组进行对比研究。结果①TIMP-2组肌成纤维细胞的α-平滑肌肌动蛋白显著高于PBS组和MMP-2组(P<0.05),且PBS组高于MMP-2组,差异有统计学意义(P<0.05)。②TIMP-2组创面平均愈合时间与PBS组、MMP-2组相比明显缩短(P<0.05),且PBS组短于MMP-2组,差异有统计学意义(P<0.05)。③MMP-2组瘢痕厚度明显小于TIMP-2组、PBS组(P<0.05),且TIMP-2组与PBS组相比有差异(P<0.05)。结论大鼠创面局部使用MMP-2会导致创面延期愈合,但愈合后瘢痕增生减轻,而使用TIMP-2会缩短愈合时间,同时会促进瘢痕增生,所以只有当MMPs-2与TIMP-2维持恰当的比例,才能发挥最好的作用。Objective To Observe the effect of exogenous matrix metalloproteinases（MMP-2） and matrix metaUoproteinase inhibitors（TIMP-2） to wound healing and the healing scar within the medical reference range of small doses. Methods Selected 32 SD rats and made the model of rat back skin wound healing,rats would be randomly divided into the MMP-2 groups, TIMP-2 groups and phosphate buffered saline（PBS） control group, and given these three reagents and compared. Results （1）The a-smooth muscle actin of fiber cells in the TIMP-2 group was significantly higher than the PBS and MMP-2 group, and it was significantly difference between the PBS and MMP-2 group（P〈0.05）; （2） Compared with the others groups, the wound average healing time in the TIMP-2 group was significantly reduced（p〈0.05）, and the PBS group was shorter than the MMP-2 group, there was statistically significant（P〈0.05）. （3）The scar thickness in the MMP-2 group was significantly lower than the TIMP-2 and PBS group（P〈0.05）, and it was significantly difference between the PBS and TIMP-2 group（P〈0.05）. Conclusion The use of MMP-2 lead to delayed healing in local wound for Rats, but.the scar formation will be reduced after healing. Reversely, the use of TIMP-2 will promote healing and scar formation, so it can play the best role only to maintain the proper proportion of MMP-2 and TIMP-2.

关 键 词：创伤愈合 瘢痕增生 基质金属蛋白酶 基质金属蛋白酶抑制剂 

分 类 号：R364.3[医药卫生—病理学]