准噶尔山楂叶总黄酮的超声波提取及抗氧化研究  

Study on the Ultrasonic Extraction and Antioxidative Activity of Total Flavonoids from Crataegus songorica K.Koch.Leaves

作　　者：任艳利[1] 张维[1] 刘影[1] 

机构地区：[1]伊犁师范学院化学与生物科学学院,新疆伊宁835000

出　　处：《安徽农业科学》2012年第15期8710-8712,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为准噶尔山楂叶的综合利用和深度开发提供一定的参考。[方法]利用超声波法提取准噶尔山楂叶中的总黄酮,用总黄酮提取率作为衡量提取工艺的指标,对影响超声波提取的多个因子进行单因素、正交试验分析。[结果]准噶尔山楂叶中的总黄酮的超声波提取最佳条件为:乙醇浓度80%,提取温度50℃,提取时间50 min,提取功率75 W,总黄酮的得率高达84.50%;影响总黄酮得率的主次因素依次为:乙醇浓度>提取时间>提取功率>提取温度。[结论]准噶尔山楂叶总黄酮有较强的清除自由基能力,表现出明显的抗氧化性。[Objective] To provide references for the comprehensive utilization and deep processing of Crataegus songorica K. Koch leaves. [ Method ] The total flavonoids were extracted from Crataegus songorica K. Koch leaves by ultrasonic wave, and the impact factors were analyzed by single factor and orthogonal experiments. [ Result] The best ultrasonic extraction conditions were 80% ethanol concentration, 50 ℃ extraction temperature, 50 min extraction time and 75 W extraction power, under the conditions, the yield of total flavonoids could reach up to 84.50% , and the influential degree of each impact factor decreased in the order of ethanol concentration 〉 ultrasonic time 〉 ultrasound power 〉 ultrasonic temperature. [ Conclusion] The total flavonoids from Crataegus songorica K. Koch leaves had strong radical scavenging ability and significant antioxidative activity.

关 键 词：超声波 准噶尔山楂叶 总黄酮 提取 抗氧化性 

分 类 号：S122[农业科学—农业基础科学]