Effect of electroacupuncture preconditioning at “Nèiguān” (内关 PC 6) on gene expression of myocardial opioid receptors in rats with ischemia-reperfusion  

电针“内关”穴预处理对缺血再灌注大鼠心肌阿片受体基因表达的影响(英文)

作　　者：李万山 钟敏 杨进辉 招伟贤 

机构地区：[1]广州中医药大学第二附属医院,中国广东广州510120 [2]广州市越秀区正骨医院

出　　处：《World Journal of Acupuncture-Moxibustion》2012年第1期43-48,共6页世界针灸杂志（英文版）

基　　金：Supported by Guangdong TCM Bureau Project: 2008115

摘　　要：Objective To explore protective effects of electroacupuncture at ＂Nèiguān＂ （内关 PC 6） for preconditioning on myocardial ischemia-reperfusion injury （MIRI） and the mechanisms involved. Methods Forty-eight male Wistar rats were randomly divided into a sham operation group （Group N）, a MIRI group （Group M） and an electroacupuncture （EA） group （Group E）. The MIRI model was established by ligating the left anterior descending artery （LAD） for 30 min followed by reperfusion for 120 min. Partition sutures were passed under LAD without ligation for rats in Group N. Rats in Group E were pretreated with electroacupuncture （EA） applied at bilateral ＂Nèiguān＂ （内关 PC 6） for 20 min once a day for 3 consecutive days before ischemia. The infarct size plus the area at risk was evaluated by 2,3,5-triphenyltetrazolium chloride staining, and serum isoenzyme of creatine kinase （CK-MB） and lactate dehydrogenase （LDH） levels were measured by biochemical methods. Myocardium morphological changes were observed under light microscopy. The mRNA expressions of myocardial δ and κ opioid receptors （DOR and KOR） were tested by real-time RT-PCR measurements. Results The myocardial infarct size in Group E was more significantly decreased than that in Group M （P0.05）. The levels of CK-MB [（980？±？92） U/L] and LDH [（2743？±？124） U/L] in Group M were significantly higher than those in Group N [（312？±？41） U/L] and [（530？±？56） U/L], respectively （both P0.01）. The levels of CK-MB [（572？±？70） U/L] and LDH [（1819？±？97） U/L] in Group E were significantly lower than those in Group M （both P0.01）. There were no significant differences in mRNA expressions of DOR and KOR between Group M and Group N （both P0.05）, but DOR expression in Group E was significantly higher than that either in Group M or in Group N （both P0.01 ）. No significant differences were found in KOR expression among the three groups （all P0.05）. Con目的:探讨"内关"穴预处理对心脏保护的效应及作用机制。方法:健康雄性Wistar大鼠48只,随机分为假手术组(N组)、心肌缺血模型组(M组)、电针组(E组)。M组采用结扎左冠状动脉前降支(LAD)30 min、再灌注120 min的方法制备心肌缺血再灌注模型; N组只开胸穿线,不结扎LAD; E组在缺血前3 d每天给予双"内关"穴电针刺激20 min。用TTC染色的方法测定大鼠心肌梗死面积;用全自动生化分析仪测定血清中肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)含量;光镜下观察心肌形态学改变;应用实时荧光定量RT-PCR法测定心肌δ阿片受体(DOR)和κ阿片受体(KOR)mRNA的表达。结果:E组较M组心肌梗死面积明显缩小(P<0.05)。M组CK-MB和LDH水平分别为(980±92)U/L和(2743±124)U/L,均较N组的(312±41)U/L和(530±56)U/L显著升高(均P<0.01),E组的CK-MB及LDH水平分别为(572±70)U/L和(1819±97)U/L,均较M组明显降低(均P<0.01)。DOR和KOR表达在M组与N组间无明显差别(均P>0.05),E组DOR表达较M组和N组显著增加(均P<0.01),KOR表达在3组间差异无统计学意义(均P>0.05)。结论:电针"内关"穴预处理可通过上调心肌δ阿片受体基因表达对缺血再灌注心肌产生保护作用。

关 键 词：ELECTROACUPUNCTURE point PC 6 （Nèiguān） ischemia reperfusion injury MYOCARDIUM receptor OPIOID 

分 类 号：R245[医药卫生—针灸推拿学]