人肝癌细胞cDNA文库的构建及鉴定  

Construction and identification of human hepatocarcinoma cell cDNA library

作　　者：黄宝成[1] 陈志南[1] 徐力青[1] 姜绍谆[2] 

机构地区：[1]第四军医大学基础部细胞工程研究中心,陕西西安710033 [2]第四军医大学基础部微生物学教研室,陕西西安710033

出　　处：《第四军医大学学报》2000年第2期138-140,共3页Journal of the Fourth Military Medical University

摘　　要：AIM To construct human hepatocarcinoma cell cDNA library. METHODS Total RNA and purified mRNA were extracted from human hepatocarcinoma cell line HHCC. The single strand and double strand of cDNA were synthesized through reverse transcription. The cDNA fragments, smaller than 500 bp, were removed and the remaining cDNAs were connected with Eco RI adaptors and then combined with the right and left arms of dephophrylated λgt11 Eco RI. The recombinant cDNAs were packaged in vitro , and then a small portion of packaged phage was used to infect E.coli Y1090 for titration. RESULTS The HHCC cell line cDNA library consisting of 1.2×10 6 recombinant bacteriophages was constructed. The average exogenous insert of the recombinants was about 1.5 kb. CONCLUSION The constructed cDNA library can be used to screen target clones.AIM To construct human hepatocarcinoma cell cDNA library. METHODS Total RNA and purified mRNA were extracted from human hepatocarcinoma cell line HHCC. The single strand and double strand of cDNA were synthesized through reverse transcription. The cDNA fragments, smaller than 500 bp, were removed and the remaining cDNAs were connected with Eco RI adaptors and then combined with the right and left arms of dephophrylated λgt11 Eco RI. The recombinant cDNAs were packaged in vitro , and then a small portion of packaged phage was used to infect E.coli Y1090 for titration. RESULTS The HHCC cell line cDNA library consisting of 1.2×10 6 recombinant bacteriophages was constructed. The average exogenous insert of the recombinants was about 1.5 kb. CONCLUSION The constructed cDNA library can be used to screen target clones.

关 键 词：肝癌细胞 CDNA文库 构建 鉴定 

分 类 号：R735.7[医药卫生—肿瘤]