牛免疫缺陷病毒反式激活因子功能域的研究  

Study of Bovine Immunodeficiency Virus Transactivator Functional Domains

作　　者：荣丽玮 王世珍[1] 刘淑红[1] 陈启民[1] 耿运琪[1] C.Wood 

机构地区：[1]南开大学生命科学院,天津300071 [2]美国Nebraska大学生命科学院

出　　处：《中国病毒学》2000年第1期88-91,共4页Virologica Sinica

基　　金：国家自然科学基金!(396 70 0 32 );美国NIH资助国际合作项目!(R0 3TW 0 0 493 0 1A1)

摘　　要：Transactivator (Tat) of bovine immunodeficiency virus (BIV) is a virus encoded regulatory protein, which activates gene expression directed by BIV long terminal repeat (LTR), and plays an important role in BIV replicative cycle. With methods of fusion protein expression and deletion mutation, a set of BIV Tat deletion mutants was constructed, and co transfected into FBL cells with BIV LTR. Using luciferase gene as a reporter, the function of BIV Tat mutants was detected and it showed that: 17/96 aa region of BIV Tat peptide contains full activity; auxiliary amino acids exist in N terminal 17/34 aa region; Cys rich region and basic region are essential to BIV Tat activity. Computer prediction of BIV Tat secondary structure was showed, and mechanism how it functions was analyzed.Transactivator (Tat) of bovine immunodeficiency virus (BIV) is a virus encoded regulatory protein, which activates gene expression directed by BIV long terminal repeat (LTR), and plays an important role in BIV replicative cycle. With methods of fusion protein expression and deletion mutation, a set of BIV Tat deletion mutants was constructed, and co transfected into FBL cells with BIV LTR. Using luciferase gene as a reporter, the function of BIV Tat mutants was detected and it showed that: 17/96 aa region of BIV Tat peptide contains full activity; auxiliary amino acids exist in N terminal 17/34 aa region; Cys rich region and basic region are essential to BIV Tat activity. Computer prediction of BIV Tat secondary structure was showed, and mechanism how it functions was analyzed.

关 键 词：牛免疫缺陷病毒 反式激活因子 缺失突变 

分 类 号：S852.65[农业科学—基础兽医学]