赖氨匹林对宫颈癌Hela细胞增殖及凋亡的影响  

Effects of Aspisol on Proliferation and Apoptosis of Hela Cells

作　　者：张乃菊[1,2] 陈天平[3] 蒋国君[2] 周家军[2] 祝晓光[2] 

机构地区：[1]蚌埠医学院第一附属医院药剂科,安徽省蚌埠市233004 [2]蚌埠医学院药学系药理教研室 [3]蚌埠医学院第一附属医院心血管内科

出　　处：《中华全科医学》2012年第6期833-834,888,F0003,共4页Chinese Journal of General Practice

基　　金：安徽省教育厅自然科学重点研究资助项目(2005KJO47ZD)

摘　　要：目的研究赖氨匹林(aspisol)对人宫颈癌Hela细胞增殖、凋亡和周期的影响。方法取处于对数生长期的Hela细胞,实验分为4组:阴性对照组只加等体积的低糖DMEM培养液Hela细胞;实验组加入不同浓度的aspisol,使其终浓度分别为1 mmol/L、5 mmol/L、10 mmol/L。采用描计细胞生长曲线法检测细胞增殖状态;HE染色、AO/EB方法进行凋亡细胞形态学的观察。Annexin V-FITC/PI双染检测赖氨匹林对Hela细胞作用24 h后细胞凋亡情况;流式细胞术分析赖氨匹林对Hela细胞作用24 h后细胞周期的变化。结果与对照组比较,aspisol(1,5,10 mmol/L)可呈时间、浓度依赖性抑制Hela细胞的增殖;HE染色光镜下见Aspisol组细胞密度减小,细胞变圆,胞核染色变浅。赖氨匹林(1,5,10mmol/L)作用24 h后诱导Hela细胞的早期凋亡率分别为(5.73±0.87)%、(19.11±2.86)%、(33.72±5.06)%,与对照组(0.46±0.69)%比较,差异有统计学意义(P<0.01),并呈浓度依赖性。细胞周期检测显示赖氨匹林对Hela细胞有G0/G1期阻滞作用。结论 aspisol对宫颈癌Hela细胞有抑制增殖、诱导其凋亡和改变其细胞周期分布,阻滞Hela细胞于G0/G1期。Objective To explore the effects of aspisol on hela cells＇ proliferation,apoptosis and cell cycle.Methods Hela cells were randomly divided into three study groups（aspisol with 1,5,10 mmol/L） and one control group.The level of cell proliferation treated with aspisol was assessed by drawing the proliferation curves of HeLa cells.Hela cells were incubated with aspisol at various concentrations for 24 h,the morphological changes of Hela cells were evaluated by HE staining and AO/EB staining,apoptosis rate in Hela cells was analyzed by Flow Cytometry（FCM）.The cell cycle of Hela cells by aspisol for 24h was analyzed by flow cytometry.Results The results of the proliferation curves demonstrated that compared with the control group,treatment with aspisol at 1 to 10 mmol/L for 1-6 days significantly inhibited growth of Hela cells in time and dose dependent manners,HE staining shows that under light microscope the cells in aspisol treatment groups showed decreased density,the cells became smaller,round shape and light staining of nucleus.Compared with the control group,the early apoptotic rates were（5.73±0.87）%,（19.11±2.86）%,（33.72±5.06）% after the exposure of the cells to aspisol at 1,5 and 10 mmol/L for 24 h respectively.Further analysis of cell cycle by flow cytometry indicated that effects of aspisol on Hela cells for 24 h led to G0/G1phase arrest.Conclusion Aspisol can inhibit cervical cancer Hela cells proliferation、induce apoptosis and prevent cell cycle progression.

关 键 词：赖氨匹林 HELA细胞 凋亡 细胞周期 

分 类 号：R737.33[医药卫生—肿瘤]