眼科疾病联合基因治疗的研究现状  

Multi-expression gene therapy of ophthalmic diseases

作　　者：王盈之[1] 魏文斌[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730

出　　处：《国际眼科纵览》2012年第2期122-127,共6页International Review of Ophthalmology

基　　金：基金项目：北京市自然科学基金项目（7092021）;国家自然科学基金项目（81041018）

摘　　要：联合基因治疗是当前多基因遗传性疾病治疗研究的热门策略，正逐渐形成一套较完整的技术理论体系。在单基因治疗研究的基础上选择一些有协同作用的基因联合治疗，取得更好疗效。在眼科，联合基因治疗遗传性视网膜色素变性、眼部新生血管性疾病、青光眼等已得到初步研究结果。本文综述了联合基因治疗的概念、优势、构建联合基因的方法、基因载体的选用以及相关实验操作，以及现阶段国内外的研究现状。The multi-expressing gene therapy is a popular strategy for the treatment research in muhi-gene genetic diseases, and it is becoming a promising technique. In order to obtain a better effect of the multi-expressing gene treatment one can use single gene therapy as a foundation and select some genes which have synergistic effect in the study of combined gene therapy. Combing the multi-expressing gcne therapy has had preliminary resuhs in some eye diseases such as inherited retinitis pigmentosa, ocular neovascular disease and glaucoma. The article reviews the concepts, advantages of the multi-expressing gene therapy, the method of combined genes construction, the selection of gene vector, the related experimental operation and the study situation at the present.

关 键 词：联合基因治疗 多基因质粒构建 病毒载体系统 非病毒载体系统 基因载体 

分 类 号：R77[医药卫生—眼科]