沙利霉素逆转P-gp介导的肾癌ACHN细胞多药耐药的实验研究  

Reversal effect of salinomycin on P-gp mediated multidrug-resistance in renal cell carcinoma line ACHN

作　　者：王可举 周辉良[2] 李斌[1] 

机构地区：[1]福建医科大学第一临床医学院,福州350005 [2]福建医科大学附属第一医院泌尿外科

出　　处：《现代泌尿生殖肿瘤杂志》2012年第2期94-96,100,共4页Journal of Contemporary Urologic and Reproductive Oncology

摘　　要：目的探讨沙利霉素对肾癌ACHN细胞多药耐药的逆转及其机制。方法实验分为对照组、阿霉素组、沙利霉素组及阿霉素和沙利霉素联合用药组,药物作用24h后,CCK-8方法检测肾癌细胞的生长活性,免疫细胞化学法检测肾癌细胞P-糖蛋白(P-gp)的表达情况。结果 10μg/ml阿霉素对肾癌ACHN细胞的生长抑制率仅为4.758%。沙利霉素组对肾癌ACHN细胞生长抑制率呈剂量依赖性,并高于阿霉素组(P<0.05),其中以10μmol/L组抑制率最高(达17.555%)。联合用药组的生长抑制率高于沙利霉素组及阿霉素组(P<0.05),以10μmol/L沙利霉素+10μg/ml阿霉素组的抑制率最高(达45.447%)。与阿霉素组比较,经沙利霉素处理过的肾癌ACHN细胞中的P-gp蛋白表达下降(P<0.05)。结论沙利霉素增强了肾癌ACHN细胞对阿霉素的敏感性,耐药性逆转的机制之一可能与沙利霉素下调癌细胞中P-gp的表达有关。Objective To investigate the reversal effect and mechanism of salinomycin on human multidrug resistant renal cell carcinoma line ACHN.Methods Four groups were designed in this experiment：control,adriamycin,salinomycin,and adriamycin ＋ salinomycin（combination group）.The cellular viability was measured by CCK-8 assay after 24 h since drug worked.Immunocytochemistory was used to test the expression of P-glycoprotein（P-gp）.Results The growth inhibition rate of 10 μg/ml adriamycin on ACHN cells was only 4.758%.The salinomycin group presented a dose-dependent effect,whose inhibition rate was higher than adriamycin group（P〈0.05）,in which the 10 μmol/L dose of salinomycin reached the highest rates（up to 17.555%）.The growth inhibition rate of combination group was higher than either of salinomycin and adriamycin group（P〈0.05）,respectively.The top rate from 10 μmol/L adriamycin ＋ 10 μg/ml salinomycin group was 45.447%.Compared with adriamycin group,expression of P-gp in ACHN cells was obviously decreased in salinomycin groups（P〈0.05）.Conclusions Salinomycin enhanced the sensitivity of renal cell carcinoma ACHN cells tagetting to adriamycin.The down-regulated expression of P-gp might be one of the mechanisms of multidrug resistance reversal.

关 键 词：沙利霉素 P糖蛋白 肾细胞癌 多药耐药 

分 类 号：R737.11[医药卫生—肿瘤]