过氧化物酶体增殖物激活受体γ在HBV、HCV感染的肝细胞癌组织中表达的研究  

Studies on the Expression of Peroxisome Proliferator-activated Receptor γ(PPAR-γ)in Human Hepatocellula Carcinoma Between HBV-Related and HCV-Related Infections

作　　者：戴小明[1] 李彬鹏[1] 黄秋林[1] 贺更生[1] 罗加兴[1] 

机构地区：[1]南华大学附属第一医院普外科,湖南衡阳421001

出　　处：《中南医学科学杂志》2012年第3期253-256,共4页Medical Science Journal of Central South China

基　　金：衡阳市科技局课题(课题编号:2011KJ26)赞助

摘　　要：目的探讨过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorγ,PPAR-γ)在HBV、HCV感染的肝细胞癌(hepatocellula carcinoma HCC)组织及相应癌旁组织中的表达情况。方法收集经手术切除且有完整临床病理资料的40例HBV阳性及16例HCV阳性的HCC组织及相应癌旁组织标本,采用免疫组织化学法检测标本中PPAR-γ的表达,用图像分析系统测定PPAR-γ的灰度值并分析其与临床病理资料的关系。结果 HBV阳性及HCV阳性HCC组织和其相应的癌旁组织中PPAR-γ的灰度平均值分别为91.13±3.28、114.18±3.27及99.75±4.99、117.69±4.91;两两比较差异均有显著性(P<0.05)。HCC组织中PPAR-γ的表达程度与患者性别、年龄、肿瘤大小、血清AFP水平、门/腔静脉癌栓有无等因素无关(P>0.05),与组织分化程度及肝炎病毒感染有关(P<0.05)。结论检测PPAR-γ对判断其恶性程度可能有一定参考价值;其可能是导致HBV阳性的HCC较HCV阳性的HCC发病率高、进展快的一个影响因素。Objective To investigate the different expression of PPAR-γ in Human HCC tissues and the corresponding adjacent nontumoral liver tissues with HBV-Related and HCV-Related infections. Methods Collect HCC tissues and the corresponding adjacent nontumoral liver tissues of 40 cases with HBV-Related infection, and 16 cases with HCV-Related infection,which were treated by surgical resection and had complete clinieopathological informations from October 2007 to October 2010. Detect the expression of PPAR-γ in the specimens by immunohistoehemistry,make a quantitative analysis of the expression of PPAR-γ, by accessing their mean gray scale with imaging analysis system, and then analyse the relationship between PPAR-γ and the clinicopathologieal informations. Results The mean gray scale of HCC tissues and the corresponding adjacent nontumoral liver tissues between cases with HBV-Related infection and with HCV-Related infection were respectively 91.13 ± 3.28 and 114.18± 3.27,99.75± 4.99 and 117.69 ± 4.91, the differences between them are very significant（ P 〈 0.05 ）. The expression degree of P PPAR-γ, in HCC tissues was not associated with sex, age ,tumor size ,serum AFP levels ,tumor embolus of portal vein or vena eana（ P 〉0.05 ） ,but with differentiation grades and hepatitis virus infection（ P 〈 0.05 ）. The differentiation grades between HCC tissues with HBV-Related infection and HCC tissues with HCV- Related infection have no significant difference（ P 〉 0.05 ）. Conclusions Detecting the expression of PPAR-γ may be valuable for evaluating malignancy extent. PPAR-γ may be a factor for which HCC with HBV-Related infection has a high incidence and a fast progress than HCC with HCV-Related infection.

关 键 词：肝细胞癌 PPAR-Γ 免疫组织化学 HBV HCV 

分 类 号：R735.7[医药卫生—肿瘤]