茎瘤芥(榨菜)高效离体再生体系的建立  

Establishment of High-frequency in vitro Regeneration System in Tuber Mustard

作　　者：沈进娟[1] 范永红[1] 冷容[1] 李娟[1] 冉广葵[1] 

机构地区：[1]重庆市涪陵区农业科学研究所、南方芥菜品种改良与栽培技术国家地方联合工程实验室、榨菜品种改良栽培技术重庆市工程实验室,涪陵408000

出　　处：《生物技术通报》2012年第5期71-75,共5页Biotechnology Bulletin

基　　金：重庆市科技攻关项目(CSTC;2010AA1023)

摘　　要：利用离体培养技术,以榨菜的3个主栽品种的子叶、带柄子叶和下胚轴为外植体,对影响榨菜植株再生的关键因素进行了优化。结果表明,培养25 d不定芽的分化率最高;最佳不定芽诱导的激素组合为6-BA 2 mg/L+NAA 0.2 mg/L,其中带柄子叶不定芽的分化率达94.4%;"蔺市草腰子"和"郫县榨菜"容易诱导不定芽,而"永安小叶"不容易被诱导,说明基因型的影响比较大;不定根的最佳诱导激素为NAA 0.2 mg/L,其生根率为83.4%。榨菜高效离体再生体系的成功建立,为榨菜遗传转化、突变体筛选和种质资源保存打下了基础。The key factors that affected tuber mustard （ Brassica juncea vat. tumida Tsen ＆ Lee ） plant regeneration were optimized by in vitro culture technology, with the cotyledons, cotyledons with petiole, and hypocotyls of three main cultivated varieties of tuber mustard as explants. The results showed that, the differentiation rate of adventitious buds was the highest when explants were cultivated for 25 days. A fairly high shoot differentiation rate （ 94.4% ） was obtained when cotyledons with petiole were cultivated on the phytohormone combination of 6-BA 2 mg/L ＋ NAA 0.2 mg/L, which was optimized for shoot induction. The frequency of shoot regeneration was significantly affected by the genotype, for example, multiple shoots were easily induced in ＂Lin-shi Cao-yao-zi＂ and ＂Pi-xian Zha-cai＂ but hardly induced in ＂Yong-an Xiao-ye＂ . The appropriate phytohormone combination for adventitious root differentiation was NAA 0.2 mg/L, with the rooting rate reaching 83.4%. The study had successfully established a highly-efficient in vitro regeneration system in tuber mustard, which laid the foundation for genetic transformation, mutant screening and germplasm resources conservation of mustard tuber.

关 键 词：离体培养 榨菜 外植体 诱导 高效再生体系 

分 类 号：S637.3[农业科学—蔬菜学]