抗衰老保健食品中白藜芦醇和辅酶Q_(10)的含量测定  

Determination of Resveratrol and Coenzyme Q_(10) in Anti-aging Health-care Food

作　　者：姜伟 杨小妹[2] 陆豫[3] 余勃[3] 

机构地区：[1]南昌市食品药品检验所,江西南昌330038 [2]深圳市蛇口人民医院,广东深圳518067 [3]南昌大学中德联合研究院,江西南昌330038

出　　处：《广州化工》2012年第10期121-122,共2页GuangZhou Chemical Industry

摘　　要：建立了同时测定抗衰老保健食品中白藜芦醇和辅酶Q10的含量的HPLC方法。采用Welch materials C18液相色谱柱,甲醇∶异丙醇(55∶45)为流动相,流速设定为1 mL·min-1,检测波长为PDA定时波长(0.00,306.0)(6.00,306.0)(6.01,275.0)(20.00,275.0),实现了白藜芦醇和辅酶Q10的良好分离分析。白藜芦醇的线性范围为0.52～156.00μg·mL-1,r=0.9999;辅酶Q10的线性范围为0.49～195.52μg.mL-1,r=0.9999。平均加标回收率及RSD分别为97.70%(RSD 0.6%)和97.40%(RSD 0.4%)。本方法灵敏度高、选择性好、操作简单,可方便地用于检测抗衰老类保健食品中白藜芦醇和辅酶Q10的含量。A high performance liquid chromatographic（HPLC） method was established for the simultaneous determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.The determination of resveratrol and Coenzyme Q10 by HPLC was performed with Welch materials ODS C18 and the mobile phase of methanol：isopropanol（55：45） at a flow rate of 1.0 mL·min^-1,and the detection wavelength was conducted at（0.00,306.0）,（6.00,306.0）,（6.01,275.0）,and（20.00,275.0）.Results showed that a good separation of resveratrol and Coenzyme Q10 was observed.The calibration curve of resveratrol was linear within the range of 0.52~156.00 μg·mL-1 and r=0.9999.The calibration curve of Coenzyme Q10 was linear within the range of 0.49~195.52 μg·mL^-1 and r=0.9999.The average recovery rate of resveratrol was 97.70% and the RSD was 0.6%.The average recovery rate of Coenzyme Q10 was 97.40% and the RSD was 0.4%.The HPLC method was characterized by high sensitivity,good selectivity,simple operation of determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.

关 键 词：抗衰老 高效液相色谱法 白藜芦醇 辅酶Q10 

分 类 号：TS207[轻工技术与工程—食品科学]