TRAIL基因多态性与卵巢癌的相关性探讨  

Investigation on the correlation between TRAIL gene polymorphism and ovarian cancer

作　　者：闫晓华 

机构地区：[1]山东医学高等专科学校,山东临沂276000

出　　处：《山东医药》2012年第19期4-6,共3页Shandong Medical Journal

基　　金：山东省高等学校科技计划项目(J10LC24)

摘　　要：目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因第五外显子3'-UTR区多态性与卵巢癌发生的相关性。方法选择175例上皮性卵巢癌患者(观察组)和170例健康体检者(对照组),采用PCR-RFLP法分析两组TRAIL基因第五外显子3'-UTR区1525G/A、1595C/T位点的基因型,比较观察组和对照组及观察组中高分化、中分化、低分化卵巢癌患者基因型和等位基因频率。结果观察组GG/CC、GA/CT基因型频率明显高于对照组,AA/TT频率明显低于对照组,P均<0.05;观察组G/C等位基因频率明显高于对照组,A/T频率明显低于对照组,P均<0.05。观察组高分化、中分化、低分化基因型频率及等位基因频率两两比较均有统计学差异,P均<0.05。结论 TRAIL基因第五外显子3'-UTR区1525G/A、1595C/T位点基因多态与卵巢癌的遗传易感性相关,携带1525G、1595C基因者卵巢癌发病风险增加。Objective To explore the association of tumor necrosis factor related apoptosis inducing ligand （TRAIL） gene polymorphism in the 3＇-untranslated region （3＇-UTR） of exon 5 with the occurrence of ovarian cancer. Methods T oral of 175 patients with epithelial ovarian cancer（ observation group） and 170 normal controls （control group）were selected, polymorphism of TRAIL gene in the 3＇-UTR of exon 5 at positions 1525G/A, 1595C/T were analyzed by PCR-RFLP method, the genotypes and allele frequencies were analyzed between observation group and control group, and also were analyzed among well-differentiated, moderately differentiated, poorly differentiated cases in observation group. Results The frequencies of GG/CC, GA/CT genotypes in observation group were significantly higher than those in control group, the frequency of AA/TT genotypes in observation group was significantly lower than that in control group, all P 〈 0.05 ; the frequency of G/C allele in observation group was significantly higher than that in control group, the frequency of A/T allele in observation group was significantly lower than that in control group, all P 〈 0.05. Significant differences in distribution of genotype and allele were shown among well-differentiated, moderately differentiated, poorly differentiated cases in obser- vation group, all P 〈0.05. Conclusion Polymorphisms of TRAIL gene 1525G/A, 1595C/T positions in 3＇-UTR of exon 5 are associated with susceptivity of epithelial ovarian cancer, and people carrying 1525 G, 1595 C gene have higher morbid- ity risk of ovarian cancer.

关 键 词：卵巢肿瘤 卵巢癌 肿瘤坏死因子相关凋亡诱导配体 基因多态性 

分 类 号：R737.31[医药卫生—肿瘤]