多药耐药相关蛋白1在三种乳腺癌细胞系中表达差异的研究  

Differential expression of MRP1 in three breast cancer cell lines

作　　者：谌海燕[1] 王荣华[1] 陈信义[2] 

机构地区：[1]北京中医药大学东直门医院血液肿瘤科,100700 [2]北京市与教育部重点实验室（中医内科学）

出　　处：《肿瘤研究与临床》2012年第4期229-231,242,共4页Cancer Research and Clinic

基　　金：北京中医药大学自主创新课题（2009TYBZZ-XS057） （谌海燕、王荣华、陈信义）;

摘　　要：目的比较多药耐药相关蛋白1（MRP1）在人类乳腺癌敏感细胞（MCF-7／S）和耐药细胞（MCF-7／TAM、MCF-7／ADR）中的表达差异。初步探索乳腺癌细胞对三苯氧胺（TAM）和多柔比星（ADR）的耐药性的发生机制。方法分别采用实时荧光定量PCR、Westernblot技术检测MRP1基因和MRPI蛋白在3种乳腺癌细胞中的表达差异。结果MRP1mRNA在MCF．7／TAM和MCF-7／ADR细胞中表达量分别是MCF-7／S细胞的（2．63±0．18）倍和（8．38±0．76）倍，差异均有统计学意义（P=O．004，P=0．003）。MRP1蛋白在MCF．7／TAM细胞和MCF-7／ADR细胞中表达均高于MCF-7／S细胞，与MRPImRNA的趋势相一致。结论MRP1的高表达可能是乳腺癌细胞对TAM和ADR产生耐药的共同发生机制。Objective To compare the differential expression of MRP1 in human breast cancer sensitive cells （MCF-7/S）and resistant cells （MCF-7/TAM,MCF-7/ADR）, and to preliminary distinguish whether there is a common mechanism between breast cancer cells resistant to tamoxifen （TAM） and to adriamycin（ADR）. Methods We detected the expression of MRP1 gene and MRP1 protein in the three breast cancer cells by using the methods of real-time fluorescence quantitative PCR, and Western blot respectively. Results The expression of MRPI gene in MCF-7/TAM and MCF-7/ADR cells was （2.63 ± 0.18） and （8.38 ± 0.76） times of MCF-7/S cells respectively, which was significantly different （P1=0.004,P2=0.003）. The expression of MRP1 protein in MCF-7/TAM and MCF-7/ADR cells was higher than MCF-7/S cells, which was consistent with the trend of MRPI gene. Conclusions The high expression of MRP1 may be the common mechanism in breast cancer cells resistant to TAM and ADR.

关 键 词：乳腺肿瘤 抗药性 肿瘤 多药耐药相关蛋白质类 

分 类 号：R737.9[医药卫生—肿瘤]