壬基酚对波纹巴非蛤(Paphia undulata)内脏团毒性效应  

Toxic effect of nonylphenol on Paphia undulata visceral mass

作　　者：巩秀玉[1,2] 陈海刚[1] 张喆[1] 马胜伟[1] 黄志斐[1,3] 王贺威[1,3] 王涛 蔡文贵[1] 

机构地区：[1]农业部南海渔业资源环境科学观测实验站,广东省渔业生态环境重点实验室,中国水产科学研究院南海水产研究所,广州510300 [2]上海海洋大学水产与生命科学学院,上海201306 [3]上海海洋大学海洋科学学院,上海201306 [4]青岛中油华东院安全环保有限公司,山东青岛266071

出　　处：《生态学杂志》2012年第6期1473-1479,共7页Chinese Journal of Ecology

基　　金：广东省科技计划项目(2009B030600001;2011A06092006);中央级公益性科研院所基本科研业务费专项资金项目(2009YD01;2010YD04;2010TS12);广东省自然科学基金博士启动项目(S2011040005251);中国水产科学研究院基本科研业务费专项课题(2012A0203)资助

摘　　要：采用半静态毒性实验方法测定了壬基酚(NP)对波纹巴非蛤(Paphiaundulata)的96-hLC50值,同时研究了低、中、高3个浓度(1、10和25μg·L-1)NP胁迫下以及胁迫解除后波纹巴非蛤内脏团中超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化物酶(POD)以及丙二醛(MDA)含量的变化趋势。急性毒性实验结果表明,NP对波纹巴非蛤的96-hLC50值为260μg·L-1。胁迫初期,低、中浓度组的SOD活性被极显著抑制(P<0.01),而POD活性则被极显著诱导(P<0.01),表现为典型的"毒性兴奋效应"。胁迫过程中,低、中浓度组波纹巴非蛤内脏团的SOD活性和GSH含量呈先下降后上升的趋势,而POD活性和MDA含量则呈先上升后下降的趋势;高浓度组SOD活性呈先抑制后诱导的趋势,POD活性和MDA含量呈先下降后升高再降低的趋势,而GSH含量则一直显著高于对照组。GSH和MDA含量在整个胁迫期间均出现剧烈的波动,且浓度越高其变化程度越大。胁迫解除后,低、中浓度组的各种指标逐渐恢复到对照组水平,但高浓度组与对照组仍存在着极显著差异。上述结果表明,NP对波纹巴非蛤内脏团的抗氧化酶系统造成较为明显的影响,而波纹巴非蛤则对一定程度的NP胁迫所带来的氧化损伤具有自我修复的能力,但对高浓度NP胁迫所造成的脂质过氧化损伤短期内却无法消除。A semi-static toxicity test was conducted to determine the 96-h median lethal concen- tration （96-h LC50 value） of nonylphenol （NP） to Paphia undulata. In the meantime, the varia- tions of superoxidc dismutase （SOD）, glutathione （GSH）, and peroxidase （POD） activities and malondialdehvde （MDA） content in the early stage of stress, the SOD activity in the visceral mass of P. undulata exposed to low and me- dium NP concentration was inhibited （P〈0.01） while the POD activity was induced （ P〈0.01 ）, showing the typical ＂excitotoxicity effect＂. During the stress period, the SOD activity and GSH content in the visceral mass at low and medium crease, while the POD activity and MDA content NP concentrations increased after an initial de- were in adverse. When exposed to high NP con- centration, the SOD activity was inhibited first and induced then, the POD activity and MDA content increased after an initial decrease, while the GSH content was significantly higher than that of the control level in whole exposure time. The GSH and MDA contents in whole exposure time appeared sharp fluctuation, and the variation During recovery period, all the test indices in the increased with increasing NP concentration. visceral mass of P. undulata exposed to low and medium NP concentration recovered gradually to the normal level, but, for the P. undulata exposed to high NP concentration, these indices remained significant difference, as compared to the control group. The above results indicated that NP had obvious impact on the antioxidant sys- tem in the visceral mass of P. undulata, while P. undulata had self-repairing ability for the oxi- dative damage caused by certain degree of NP stress peroxidation damage could not recover to the level of , but, at high NP concentration, the lipid the control group in a short time.

关 键 词：壬基酚 波纹巴非蛤 内脏团 96h半致死浓度 抗氧化防御系统 

分 类 号：X171.5[环境科学与工程—环境科学]