DMSO诱导下蛋白激酶C与HL-60细胞分化的关系  

Correlation between Protein Kinase C and Differentiation of HL-60 Cells Induced by DMSO

作　　者：汪黎[1] 糜静娴[1] 范启修[1] 

机构地区：[1]中国医学科学院血液学研究所

出　　处：《中国医学科学院学报》1990年第2期96-100,共5页Acta Academiae Medicinae Sinicae

摘　　要：HL-60细胞在DMSO诱导下沿粒细胞方向分化,120h NBT^+细胞达84％。细胞经诱导24h蛋白激酶C活性增加,72h为未诱导细胞的2.7倍。酶活性的增加,主要是由于细胞浆部分而不是细胞膜部分可溶性酶活性改变的结果。分化诱导剂DMSO本身不能直接激活蛋白激酶C,提示DMSO对酶活性的影响是间接的。Treatment of HL-60 cells with DMSO caused cells to differentiate along the myeloid way and 84% of the cells showed a reduction in NET after 120h. Protein kinase C activity increased of during differentiation. It would reach 2.7 times that of the control after 72h in the presence of DMSO. The increase of protein kinase C activity was traced to the cytosolic rather than the membrane fraction. Chelator EGTA was necessary to extract protein kinase C. DMSO itself could not enhance protein kinase C activity, indicating that the effect of DMSO was indirect. All of these results showed that the activation of protein kinase C is important in the differentiation of HL-60 cells.

关 键 词：蛋白激酶C HL-60细胞 二甲基亚砜 

分 类 号：R362[医药卫生—病理学]