粘性放线菌5951菌毛亚单位组成分析  

A Preliminary Study on Fimbriae Subunits and Molecular Weight of Actinomyces Viscosus 5951

作　　者：朱敏[1] 黄冬生 周芸 梁景平[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔内科,上海200011

出　　处：《上海口腔医学》2000年第1期2-4,共3页Shanghai Journal of Stomatology

基　　金：国家自然科学基金!资助课题 (编号 :3 9670 782 )

摘　　要：目的　为深入研究粘性放线菌Ⅱ型菌毛的粘附功能 ,对仅含Ⅱ型菌毛的T14V变异菌株 5 95 1细菌Ⅱ型菌毛的分子质量及亚单位组成进行初步分析。方法　采用免疫印迹实验对纯化的Ⅱ型菌毛进行分子质量检测。结果　显示分子质量为 31ku一条单一的色带 ,表明粘放菌 5 95 1菌毛是由相同的分子质量为 31ku的亚基组成。结论　根据文献报道及本实验结果推测 ,粘放菌 5 95 1菌毛分子质量为 12 4ku ,由 4个相同亚基组成。Objective To preliminarily analyse the subunits and molecular weight of fimbriae of actinomyces viscosus 5951. Methods To do the immunoblotting test of the only Ⅱ fimbriae type of mutant strain of T14V. Results It showed that there was only one 31ku band,which indicated that the fimbriae of actinomyces viscosus 5951 was composed of the same subunits of 31ku molecular weight. Conclusion According to the previous reports and results of present study,the molecular weight of fimbriae of actinomyces viscosus 5951 was speculated to be 124ku and the fimbriae was composed of 4 same subunits.

关 键 词：Ⅱ型菌毛 亚单位 粘性放线菌 5951菌 

分 类 号：R780.2[医药卫生—口腔医学]