载脂蛋白E基因多态性对血脂水平的影响及与冠状动脉狭窄的关系  

Effect of Apolipoprotein E Polymorphisms on Serum Lipids and Relation to Coronary Stenosis

作　　者：李卫华[1] 黄德嘉[2] 杜修海[1] 张卫泽[1] 胡静泠[1] 

机构地区：[1]兰州军区总医院心内科,兰州730050 [2]华西医科大学附属第一医院心内科,成都610041

出　　处：《中国动脉硬化杂志》2000年第1期54-57,共4页Chinese Journal of Arteriosclerosis

摘　　要：为探讨载脂蛋白E基因多态性对血脂水平的影响及与冠状动脉狭窄程度的关系 ,采用聚合酶链反应—限制片段长度多态性对 95例冠心病患者和 46例正常对照者载脂者蛋白E基因型进行分析 ,同时测定血脂及载脂蛋白B水平。并根据冠状动脉受累支数不同将冠心病患者分为冠状动脉多支病变组和单支病变组。结果显示 ,5 5例冠状动脉病变多支组和 40例冠状动脉单支病变组E3/ 4基因型和ε4等位基因频率均高于对照组 (P <0 .0 1) ,且多支病变组E3/ 4基因型和ε4等位基因频率高于单支病变组 (P <0 .0 5 )。与E3/ 3及E2 / 3基因型比较 ,E3/ 4基因型者有较高的总胆固醇、低密度脂蛋白胆固醇和载脂蛋白B水平及较低的高密度脂蛋白胆固醇水平。与对照组比较 ,冠心病组E3/ 4基因型升高总胆固醇、低密度脂蛋白胆固醇和载脂蛋白B的作用及降低高密度脂蛋白胆固醇的作用更明显。表明载脂蛋白E基因多态性影响血胆固醇代谢 ,ε4等位基因与冠心病危险性增加有关 ,ε4等位基因频率升高的冠心病者冠状动脉受累支数加重 ,推测ε4等位基因可能与冠状动脉狭窄程度存在内在联系。Aim To evaluate the effect of apolipoprotein E (apo E) polymorphism on serum lipids and the relationship between apo E polymorphism and coronary stenosis. Methods The polymorphis site of apo E gene were studied using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 95 cases with coronary artery disease (CAD) and in 46 controls of equivalent age. The serum lipids and apolipoprotein B (apo B) levels were measured in the two groups. Results The frequencres of apo E3/4 genotype and apo E4 allele were significantly higher in 55 cases of CAD with multiple vessel dissease (MVD) and in 40 cases of CAD with single vessel disease (SVD) than in 46 controls (P<0.01), while there were significantly higher in the MVD groups than in the SVD groups (P<0.05). Apo E3/4 genotype had significantly higher serum total cholesterol (TC), LDL-cholesterol (LDLC) and apo B levels and lower HDL-cholesterol (HDLC) level compared to apo E3/3 and apo E2/3 genotypes in the patients and in the controls. In the patients , apo E3/4 genotype had significantly higher TC, LDLC and apo B levels and lower HDLC levels compared with in the controls . Conclusions It is concluded that apoE polymorphism is involved in cholesterol metabolism , where apo E4 allele is associated with an increased risk of CAD. In CAD cases , an increased frequency of apo E4 allele is associated with an increased severity of coronary stenosis. Therefore it is assumed that there is certain relation between apoE4 allale and severity of coronary stenosis.

关 键 词：载脂蛋白E 基因 多态性 冠状动脉再狭窄 

分 类 号：R363.2[医药卫生—病理学] R543.302[医药卫生—基础医学]