一株提高刺五加苷E含量内生真菌的鉴定及其作用机制初探  

Identification of an Endophytic Fungi and Its Effect on Eleutheroside E Content

作　　者：邢朝斌[1] 何闪[1] 熊亚南[1] 吴鹏[1] 王明艳[1] 陈龙[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000

出　　处：《时珍国医国药》2012年第5期1092-1094,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河北省自然科学基金(No.C2009001252)

摘　　要：目的确定分离自刺五加的内生真菌P116-1a的分类地位,并初步明确其提高刺五加苷E含量的作用机制。方法应用形态学及ITS序列分析方法进行鉴定,将菌液回接刺五加浸出液进行发酵培养和将灭活后的菌液注射刺五加后,HPLC法分析刺五加苷含量的变化。结果 P116-1a与Penicillium minioluteum的形态学特征相符,且与其ITS序列同源性最高(98.6%)。在培养早期,活体P116-1a可显著提高刺五加浸出液中刺五加苷E的含量,灭活后其对刺五加苷E含量无显著作用。结论 P116-1a为Penicillium minioluteum,其作用方式为通过调节刺五加苷生物合成关键酶的表达、酶蛋白的活性而提高刺五加苷E的含量。Objective To identify endogenous fungi P116 -1 a isolated from Eleutherococcus senticosus, and to study its effect on eleutheroside content. Methods P116 - 1 a was identified by morphological characteristics and ITS sequencing. After P116 - 1 a and inactivated Pl16 - la was injected into E. senticosus, the content change of eleutheroside was analyzed by HPLC. Results P116 - 1 a correspond to morphological characteristic of Penicillium minioluteum, and had the highest homology with Penicillium minioluteum（ 98. 6% ）. In the early cultivation phase, P116 - 1 a improved eleutheroside E significantly, however the inactivated P116 - 1 a didn＇t improve eleutheroside content. Conclusion P116 - 1 a confirmed as Penicillium minioluteum. Penicillium minioluteum can regulate expression or increase activity of biosynthesis key enzyme to increase eleutheroside content.

关 键 词：内生真菌 刺五加苷 青霉 

分 类 号：R282.5[医药卫生—中药学] R285.5[医药卫生—中医学]