热休克对T淋巴细胞专一mRNA表达的影响  

The Effect of Heat Shock on mRNA Expression in Human T Lymphocytes

作　　者：刘泽津 吴宁华 沈珝琲 

机构地区：[1]中国协和医科大学 [2]基础医学研究所

出　　处：《中国医学科学院学报》1990年第6期416-420,共5页Acta Academiae Medicinae Sinicae

摘　　要：分别提取热休克前后的激活淋巴细胞及Jurkat细胞的总(?)mRNA,以之为模板通过逆转录法得到以α-^(32)P标记的第一链cDNA,再以cDNA为探针同abl,myc,myb,fos,ras五种癌基因及TcR-β,IL-2,IL-2R,TfR四种与细胞生长增殖密切相关的基因片段进行斑点杂交。结果表明热休克可使激活的淋巴细胞和Jurkat细胞中c-myc和TfR mRNA的表达明显降低,而IL-2和Ki-rat的表达增加。并对热休克与基因表达的关系进行了初步讨论。Total mRNA was extracted from activated T lymphocytes and Jurkat cells with and without heat shock, and then used for α^(-32)P—labeled 1st strand cDNA synthesis with reverse transcriptase. DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras)and of IL-2, IL-2 receptor, T-cell receptor β-chain and transferrin receptor were dotted onto nitrocellulose filters. Hybridization results showed that the expression of c-myc and TfR mRNA was much lower in heat-shocked cells than in their normal counterparts. However IL-2 and Ki-ras mRNA increased after heatshock. Possible explanations for the results are discussed.

关 键 词：热休克 T细胞 基因表达 癌症 

分 类 号：R730.59[医药卫生—肿瘤]