应用SELDI-TOF-MS技术筛选肝癌血清蛋白标记物  

SELDI-TOF-MS screening serum protein markers in hepatocellular carcinoma

作　　者：苏乃伦[1] 牟文凤[1] 王斌[2] 

机构地区：[1]青岛市妇女儿童医疗保健中心检验科,山东青岛266011 [2]青岛大学医学院微生物教验室,山东青岛266071

出　　处：《中国现代医学杂志》2012年第13期53-56,共4页China Journal of Modern Medicine

摘　　要：目的运用表面增强激光解吸附/离子化时间飞行质谱技术(SELDI-TOF-MS)检测肝癌患者血清蛋白质的特异性生物标志,建立筛选血清蛋白质图谱模型并初步验证。方法选择肝癌患者30例,HBV阳性携带者20例及正常人20例,应用SELDI-TOF-MS技术及相应的计算机软件进行比较分析,检测肝癌血清蛋白质的特异性生物标志,建立肝癌的筛选模型,并对其进行了双盲法验证。结果肝癌组与对照组共有25个蛋白质差异有显著性,以其中5个蛋白标志物(6 888、13 764、6 487、3 450和6 814 Da)建立的筛选模型检测灵敏度为96.7%(29/30),特异性为90.0%(36/40),盲法验证,灵敏度为95.5%(21/22),特异性为90%(27/30)。结论SELDI-TOF-MS技术具有高灵敏度和特异性,在肝癌的诊断及肿瘤特异性生物标志的筛选等方面具有一定价值。【Objective】 To detect specific biomarkers in serum of hepatocellular carcinoma（HCC） patients using Surface Enhanced Laser Desorption Inhibition Time of Flight Ionization Mass Spectrometry（SELDI-TOF-MS）,and establish a serum protein pattern for screening HCC.【Methods】 Serum samples of 30 HCC patients,20 HBV carriers and 20 healthy controls were detected by SELDI-TOF-MS.The collected data were analyzed by Biomarker Wizard and BPS software to establish a serum protein pattern for screening HCC.The pattern was evaluated by masked test.【Results】 Total 25 peaks showed significant differences between HCC patients and controls,among which 5 peaks（6 888,13 764,6 487,3 450,6 814 Da） were automatically chosen to set up a serum protein pattern for screening HCC.The sensitivity and specificity of the pattern was 96.7%（29/30） and 90.0%（36/40）.When tested by masked samples,it was 95.5%（21/22） and 90%（27/30）,respectively.【Conclusion】 SELDI-TOF-MS had high sensitivity and specificity.It would play a valuable role on the diagnosis of HCC and on the screening of tumor specific biomarkers.

关 键 词：表面增强激光解吸附/离子化时间飞行质谱技术 肝癌 生物标志 

分 类 号：R735.7[医药卫生—肿瘤]