胰腺癌组织中p53和bc1-2蛋白的表达和意义  

The expression and significance of p53 and bc1-2 Proteins in pancreatic ductal adenocarcinoma

作　　者：陈晓琴[1] 袁世珍 

机构地区：[1]贵阳医学院附院内科,550004 [2]中山医科大学孙逸仙纪念医院消化疾病研究中心

出　　处：《贵州医药》2000年第2期71-72,共2页Guizhou Medical Journal

基　　金：美国中华医学基金 (CMB)

摘　　要：目的通过检测凋亡抑制基因bc1- 2蛋白和突变型p 5 3蛋白在不同胰腺疾病组织中的表达 ,探讨其生物学意义。方法应用标记的链霉卵白素 -生物素 (LSAB)免疫组化方法对 33例胰腺导管腺癌 (PC)、10例慢性胰腺炎 (CP)、9例正常胰腺组织进行p5 3及bc1- 2癌基因蛋白检测。结果bc1- 2和p 5 3蛋白阳性表达仅见于PC ,阳性率分别为 36 %和 5 5 % ;p 5 3蛋白表达与胰腺癌临床病理特征无关 ,而bc1- 2蛋白表达在组织学 1、2级和临床 1、2期显著高于组织学 3级和临床 3、4期。结论p 5 3和bc1- 2蛋白在部分胰腺癌的发生中可能起重要作用 ;bc1- 2蛋白表达可能为胰腺导管腺癌的早期表现。Objective To study the biological significance through detection oncogene products p53 and bc1-2 protein expression in different pancreatic disease organization.Methods p53 and bc1-2 oncogene proteins were detected by labelled streptavidinbiotion (LSAB) immunohistochemistry in paraffinembedded tissues from 33 patients with pancreatic ductal adenocarcinoma (PC), 10 chronic pancreatitis (CP) and 9 normal pancreas.Results The positive expression of p53 and bc1-2 proteins were only found in PC, with positive rates 36% and 55% respectively. The expression of p53 was irrelative to the clinical and histopathologic features of PC. The expression of bc1-2 was related to histopathologic and clinical. bc1-2 positive rate was remarkable higher in 1,2 grade and 1,2 stage than in 3 grade and 3,4 stage.Conclusion The expression of p53 and bc1-2 might play an important role in the genesis of PC. The expression of bc1-2 might be an early stage event of PC.

关 键 词：胰腺癌 癌基因 癌基因蛋白 P53 免疫组织化学 

分 类 号：R735.902[医药卫生—肿瘤]