双歧杆菌对人大肠癌HT_(29)细胞谷胱甘肽巯基转移酶基因的诱导表达  

GENE INDUCTIVE EXPRESSION OF BIFIDOBACTERIUM ON GLUTATHIONE S-TRANSFERASE IN HT_(29) CELL LINE

作　　者：唐立[1] 赵宝昌[1] 崔秀云[2] 

机构地区：[1]大连医科大学微生态研究所,大连116027 [2]大连医科大学生化教研室

出　　处：《中国微生态学杂志》2000年第1期17-18,共2页Chinese Journal of Microecology

基　　金：国家自然科学基金

摘　　要：应用北杂交技术研究双歧杆菌对大肠癌HT29细胞谷胱甘肽巯基转移酶基因的诱导表达发现:双歧杆菌及其发酵过滤液在作用HT29细胞2小时后,谷胱甘肽巯基转移酶基因的mRNA分别提高1.4倍和1.3倍,提示诱导谷胱甘肽巯基转移酶基因的高效表达,阻断外来致癌物的亲核攻击可能是双歧杆菌抗肿瘤的一个分子机制。The Northern hybridization technique was applied to study the effect of Bifidobacterium on the inductive gene expression of glutathione S-transferase. It has been found that the level of glutathione S-transferase (GSTs) mRNA increases 1 4 fold, 1 3 fold respectively,when HT 29 cells were exposed 2 hours to Bifidobacterium and filtration of fermentation. It was indicated a molecular mechanism of Bifidobacterium antitumor in that GST gene is inductive expression and preventing Xenbiotics nucleophilic attack.

关 键 词：双歧杆菌 谷胱甘肽巯基转移酶 诱导表达 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]