AG490对前列腺癌DU145细胞增殖及STAT3信号通路相关蛋白表达的影响  

Effect of AG490 on DU145 cell proliferation and expressions of STAT3 signaling pathways related proteins

作　　者：张嵩[1] 郝斌[1] 李惠翔[2] 张海鹏[1] 

机构地区：[1]郑州大学第二附属医院泌尿外科,郑州450014 [2]郑州大学第一附属医院病理科,郑州450052

出　　处：《郑州大学学报（医学版）》2012年第3期342-344,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州大学研究生科学研究基金资助项目10Y03304

摘　　要：目的:观察AG490对前列腺癌DU145细胞增殖及STAT3信号通路相关蛋白表达的影响,探讨其抗癌机制。方法:DU145细胞分组培养,对照组单纯用DMEM高糖培养基培养,实验组给予终浓度为10、25、50及80μmol/L的AG490,分别培养24、48和72h后,采用MTT比色法测定细胞增殖抑制率,Western blot检测作用72h后STAT3信号通路相关蛋白(p-JAK2、STAT3、p-STAT3、CyclinD1及BCL-xL)。结果:AG490能够抑制DU145细胞的增殖,呈剂量(F=786.170,P<0.001)、时间依赖性(F=354.570,P<0.001)。AG490作用于DU145细胞72h后,p-JAK2、STAT3、p-STAT3、CyclinD1及BCL-xL蛋白的表达均降低。结论:AG490能抑制前列腺癌DU145细胞的增殖,其机制可能与阻断STAT3信号通路有关。Aim：To observe the effect of AG490 on DU145 cells proliferation and expressions of STAT3 signaling pathways related proteins,and explore its anti-cancer mechanism.Methods：DU145 cells were grouped.The control group was cultured with DMEM high sugar culture medium,and the other groups were given AG490 at 10,25,50,and 80 μmol/L and cultured separately for 24,48 and 72 h.Using MTT method to detect the cell proliferation inhibition rate,and Western blot to test the expression levels of STAT3 signaling pathways related proteins（p-JAK2,STAT3,p-STAT3,Cyclin D1 and BCL-xL）.Results：AG490 could significantly inhibit the DU145 cells proliferation in a dose-（F=786.170,P0.001） and time-dependent manner（F=354.570,P0.001）.The protein expression levels of p-JAK2,STAT3,p-STAT3,Cyclin D1 and BCL-xL obviously reduced after 72 h AG490 treatment.Conclusion：AG490 can effectively inhibit cell proliferation of DU145 cells,which may be involved in its blocking STAT3 signaling pathways.

关 键 词：AG490 前列腺癌 DU145 STAT3 增殖 

分 类 号：R737.25[医药卫生—肿瘤]