三七素对谷氨酸损伤的PC12细胞的影响  

The Effects of Dencichine on PC12 Cells Injuried by Glutamate

作　　者：姜珊[1,2] 史明[1] 张琛[1] 胡耿瑶[1] 赵钢[1] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032 [2]西安市第一医院干部病房,陕西西安710032

出　　处：《现代生物医学进展》2012年第12期2272-2274,共3页Progress in Modern Biomedicine

摘　　要：目的:研究三七素对谷氨酸损伤的PC12细胞的影响。方法:用谷氨酸复制体外培养的PC12细胞损伤模型,采用MTT法、Hoechst33342/PI双重染色法分别研究高、低剂量三七素对谷氨酸所致的PC12细胞损伤的影响。结果:谷氨酸刺激后,PC12细胞存活率较对照组显著降低(P<0.05)、凋亡显著增强(P<0.05)。给予高剂量三七素可加重谷氨酸引起的PC12增殖力降低、凋亡增强。但低剂量三七素干预后,细胞存活率较模型组显著升高(P<0.05),凋亡较模型组明显降低(P<0.05)。结论:高剂量三七素可加重谷氨酸对PC12细胞的损伤,但低剂量三七素可显著减轻谷氨酸对PC12细胞的损伤,但具体机制尚有待于进一步研究。Objective： To investigate the effect of dencichine on PC12 cells injuried by glutamate. Methods： PC12 cells were injuried by glutamate, and then the cells were theraped respectively by dencichine at the doses of 0.5 mg/kg and 0.5 mg/kg. The proliferative ability and apoptosis of PC12 cells were detected by MTT and Hoechst33342/PI. Results： Dencichine at the dose of 2.5 mg/kg aggravated the inhibition of proliferative ability and apoptosis of PC 12 cells induced by glutamate, but dencichine at the dose of 0.5 mg/kg ameliorated the inhibition of proliferative ability and apoptosis of PC12 cells induced by glutamate. Conclusions： Dencichine is neurotoxic, dencichine and low dose of which could ameliorated the impairment caused by glutamate.

关 键 词：三七素 PC12细胞 谷氨酸 

分 类 号：Q95-3[生物学—动物学] R742.1[医药卫生—神经病学与精神病学]