pH值对两种胶体金试剂条检测胃液潜血的影响  

Effects on results of occult blood test in gastric juice at different pH values by two gold immunochromatographic assay strips

作　　者：祁冬冬 陈英利[2] 李翀[3] 张涛[4] 

机构地区：[1]内蒙古林业总医院检验科,内蒙古牙克石022150 [2]黑龙江哈尔滨医科大学(大庆)医学检验与技术学院,黑龙江大庆163319 [3]宁夏石嘴山市第二人民医院检验科,宁夏石嘴山753000 [4]黑龙江佳木斯大学基础学院生物化学与分子生物学教研室,黑龙江佳木斯154000

出　　处：《中国卫生检验杂志》2012年第5期1089-1090,1093,共3页Chinese Journal of Health Laboratory Technology

基　　金：黑龙江省研究生创新科研项目专项资金(YJSCX2011-377HLJ);黑龙江省卫生厅科技项目(2009-257)

摘　　要：目的:研究在胃液潜血实验中不同pH值对结果的影响。方法:在中性环境下逐渐增加血红蛋白浓度,测定两种潜血试纸条的最低检测限;用5 mol/L NaOH调节新鲜胃液的pH值,确定能检测出血红蛋白的临界pH值;在临界pH的环境里,改变血红蛋白浓度和测定时间,检测时间与浓度对检出率的影响。结果:两种试纸条的最低检测限为0.2μg/ml和0.4μg/ml;pH值3.7时不能检测出血红蛋白;在pH<3.7的酸性环境里,血红蛋白浓度升高会暂时延长潜血实验结果阳性的时间。结论:胃液潜血实验在pH<3.7酸环境中检测,使结果出现假阴性,可以通过调节pH值和镜下观察得出正确结论。Objective：To study the influence of different PH values on the results of occult blood test in gastric juice.Methods： In the neutral environment,the minimum detection limit of two kinds of occult blood test strip was measured by gradually increasing the hemoglobin concentration;To adjust the PH value of fresh gastric juice in 5 mol/L NaOH solution and determine the critical PH value,hemoglobin can be detected at this critical PH value;at the critical PH value,hemoglobin concentration and measurement time were changed to detect their influence on the test results.Results： The minimum detection limit of two kinds of occult blood test strip respectively were 0.2 μg/ml and 0.4 μg/ml;As PH value of gastric juice was 3.7,hemoglobin can not be detected,while PH〈3.7,increase of hemoglobin concentration can tentatively extend the time for positive occult blood test results.Conclusion： In PH〈 3.7 acid environment,the occult blood test will give the false negative results.Testing personnel can draw the right conclusions by adjusting the PH value of gastric juice and using microscope to observe the form of red blood cells.

关 键 词：胃液 潜血 血红蛋白 PH值 

分 类 号：R573.9[医药卫生—消化系统]