ezrin表达与宫颈鳞癌组织微血管微淋巴管生成、癌细胞增殖、浸润转移及预后的关系  

Relationship between expression of ezrin protein and angiogenesis,lymphangiogenesis,cancer cell proliferation,invasion,metastasis and prognosis in squamous cell carcinoma of cervix

作　　者：齐碧如[1] 江忠清[2] 连成瑛[3] 黄勉[1] 林岷 刘兆董[3] 方娇宁[3] 

机构地区：[1]福州市第一医院妇产科,350009 [2]福建医科大学附属第一医院妇科 [3]福建省妇幼保健院妇产科 [4]福建医科大学基础医学院生理学与病理生理学系

出　　处：《中国实用医药》2012年第12期1-5,共5页China Practical Medicine

基　　金：福州市科技局项目(项目编号:2007-S-124);福建省教育厅项目(项目编号:JA04210;JA03104)

摘　　要：目的探讨宫颈鳞癌组织埃兹蛋白(ezrin)表达与浸润转移和预后的关系。方法采用免疫组织化学SP法检测28例正常宫颈上皮(NCE)、36例宫颈上皮内瘤变(CIN)和68例宫颈鳞癌(SCC)中ezrin表达,并检测MIB1标记的增殖指数(PI)、CD34标记的微血管密度(MVD)和VEGFR-3标记的微淋巴管密度(LMVD)。结果从NCE到CIN再到SCC,ezrin表达水平显著升高(P<0.05)。ezrin在SCC中表达与FIGO分期、盆腔淋巴结转移、间质浸润及脉管浸润有关(P<0.05),而与患者年龄及组织学分级无关(P>0.05)。FIGO分期为Ⅱ期、有盆腔淋巴结转移、突破深肌层间质浸润和有脉管浸润者,ezrin表达水平显著高于FIGO分期为Ⅰ期、无盆腔淋巴结转移、浸润深度在浅肌层间质以内和无脉管浸润者(P<0.05)。从NCE到CIN再到SCC,PI、MVD和LMVD显著升高(P<0.05)。ezrin在SCC中表达与PI、MVD及LMVD均无显著相关性(P>0.05)。宫颈鳞癌组织ezrin高表达组患者生存率明显低于低表达组,但差异无统计学意义(P=0.0631)。结论 ezrin可能在宫颈鳞癌浸润转移过程中起重要作用,并可能成为一个有价值的预后标志物。Objective To examine relationship between expression of ezrin and invasion, metastasis, and prognosis in cervical squamous cell carcinoma. Methods The expression of ezrin, proliferation index （PI） of cancer ceils labeled by MIB1, microvessel density （MVD） labeled by CD34 and lymphatic microvessel density （LMVD） labeled by VEGFR-3 protein in 28 cases of normal cervical epithelium（ NCE）, 36 cases of cervical intraepithelial neoplasm（CIN） and 68 cases of squamous carcinoma of cervix（SCC） were detected by immunohistochemistry SP method. Results The positive expression of ezrin increased remarkably from NCE to CIN and then to SCC accordingly （P 〈 0. 05）. In SCC, the expression of ezrin correlated with FIGO staging, pelvic lymph node metastasis, stroma involvement and intravascular infiltration （ P 〈 0. 05 ）, but not correlated with patient＇s age and histological grading （P 〉 0.05 ）. In cases with FIGO staging II , pelvic lymph node metastasis, deeper stroma involvement and intravascular infiltration, the expression of ezrin was significantly higher than that in those without the conditions mentioned above （P 〈 0.05 ）. The PI, MVD and LMVD all increased remarkably from NCE to CIN and then to SCC accordingly （P 〈 0.05）. The expression of ezrin in SCC was not correlated with PI, MVD and LMVD, respectively （P 〉 0.05）. The patients with higher expression of ezrin in SCC had lower survival rate than that in those without the conditions mentioned above, but the difference was not significant （P 〉 0.05 ）. Conclusion Ezrin protein may play an important role during the cancer invasion and metastasis, and may be a valuable marker in prognosis in cervical squamous cell carcinoma.

关 键 词：宫颈肿瘤/病理学 埃兹蛋白 肿瘤转移 微血管密度 微淋巴管密度 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]