食管癌组织CD15抗原和组织蛋白酶D表达的关系  

Association of CD15 antigen expression with cathepsin D in esophageal carcinoma tissues

作　　者：谷化平[1] 尚培中[2] 苏红[1] 李志钢[1] 

机构地区：[1]中国人民解放军251医院病理科,河北省张家口市075000 [2]中国人民解放军251医院普外科,河北省张家口市075000

出　　处：《世界华人消化杂志》2000年第3期259-261,共3页World Chinese Journal of Digestology

摘　　要：目的 探讨CD15抗原和组织蛋白酶D(Cath-D)在食管癌的表达意义及其相互关系。 方法 应用微波-LSAB免疫组化法,观察65例食管癌组织(男46例,女19例;平均年龄54岁;均为鳞癌)中CD15和Cath-D的表达阳性率及其相互关系。 结果 食管癌CD15和CD阳性率分别为58％(38/65例)和65％(42/65例);食管癌CD15和Cath-D表达与性别、年龄、肿瘤部位、大体类型及肿瘤大小无关(P>0.05)。CD15和Cath-D表达阳性率:鳞癌Ⅲ级(88％,14/16 vs 100％,16/16)均显著高于鳞癌Ⅰ级(43％,6/14 vs 50％,7/14)和鳞癌Ⅱ级(51％,18/35 vs 54％,19/35,P<0.05);外膜层浸润(74％,20/27 vs82％,22/27)显著高于肌层浸润(47％,18/38 vs 53％,20/38,P<0.05);有淋巴结转移组(72％,26/36 vs 78％,28/38)显著高于无淋巴结转移组(41％,12/29 vs 48％、14/29,P<0.025);死亡组(70％,31/44 vs 77％,34/44)显著高于五年生存组(33％,7/21 vs 38％,8/21,P<0.01)。CD15阳性的肿瘤Cath-D阳性率显著高于CD15阴性者(82％,31/38 vs41％,11/27),CD15表达与Cath-D表达呈正相关(r=0.42,P<0.01)。 结论 检测CD15和Cath-D表达对判断食管癌恶性程度、预测其侵袭转移趋势和预后及指导治疗有重要意义。食管癌CD15与Cath-D表达具有相互协同作用可能。AIM To study the association of CD15 antigen expression with cathepsin D(Cath-D) in esophageal carcinoma. METHODS Microwave-LSAB immunohistochemcalmethod was used to examine the expression of CD15 antigen and Cath-D in 65 specimens(46 men, 19 women, mean age was 54 years, all patients with squamous carcinoma) of esophageal carcinoma. RESULTS The positive rates of CD15 antigen and Cath-D were 58% (38/65) and 65% (42/65) respectively. The expressions of CD15 antigen and Cath-D were not related to sex,age,and tumor location. CD15 antigen and Cath-D expression rate in squamous cell carcinoma grade Ⅲ was 88% (14/16) and 100% (16/16), which was significantly higher than that in grade Ⅰ (43%, 6/14) and grade Ⅱ (51%, 18/35, P<0.05). Cath-D expression in grade Ⅲ was 100% (16/16), which was significantly higher than that in grade Ⅰ (50%, 7/14) and grade Ⅱ (54%, 19/35, P<0.05). The positive rates of CD15 antigen and Cath-D in patients with tumors invading beyond muscle were 74% (20/27) and 82% (22/27), markedly higher than 47% (18/ 38) and 53% (20/38) in patients only with muscular invasiveness (P<0.05). CD15 antigen and Cath-D expression rates in patients with lymph nodes metastasis were 72% (26/36) and 78% (28/38) respectively, which were significantly higher than 41% (12/29) and 48% (14/ 29 ) in patients without lymph nodes metastasis (P<0.025). The positive rates of CD15 antigen and Cath-D in the dead patients were 70% (31/44) and 77% (34/ 44), markedly higher than 33% (7/21) and 38% (8/21) in patients who survived more than 5 years (P<0.01). The positive rate of Cath-D in CD15 antigen positive tumors was 82% (31/38), significantly higher than that in CD15 antigen negative ones (41%, 11/27). The expression of CD15 antigen was positively correlated with the expression of Cath-D (r = 0.42, P< 0.01). CONCLUSION Detection of CD15 antigen and Cath-D expressions was important to determining the degree of malignancy and prognosis of esophageal carcinoma. There might be an interaction between CD15 antigen and Cath-D

关 键 词：食管肿瘤 代谢 CD15抗原 组织蛋白酶D 

分 类 号：R735.1[医药卫生—肿瘤]