替莫唑胺化疗对胶质瘤U251中CD133^+细胞的影响  

Effects of temozolomide chemotherapy on CD133^+ cells in glioma U251

作　　者：杨伟现[1] 向定朝[1] 王存祖[1] 周月鹏[2] 欧阳琦[2] 张志坚[2] 许慧中[1] 陆晓峰[1] 

机构地区：[1]江苏大学附属医院神经外科,212001 [2]江苏大学基础医学院

出　　处：《江苏医药》2012年第10期1124-1127,F0003,共5页Jiangsu Medical Journal

基　　金：江苏省卫生厅面上科研项目(H2006040)

摘　　要：目的探讨化疗耐药与肿瘤干细胞之间的关系。方法分析替莫唑胺(TMZ)作用前后胶质瘤U251中CD133蛋白表达及阳性细胞数量的变化。通过MTT实验评估U251细胞对TMZ的敏感性。利用免疫荧光染色和Western blot检测TMZ作用前后U251细胞中CD133、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经胶质细胞纤维酸性蛋白(GFAP)等蛋白表达。结果U251细胞中未加入TMZ组的CD133+细胞比例约为0.060±0.003,明显低于加入TMZ组的0.337±0.012(P<0.05)。在TMZ作用后,CD133和nestin表达明显增加,而GFAP和NSE表达明显减少(P<0.05)。结论胶质瘤U251细胞株中存在CD133+细胞,大部分具有脑肿瘤干细胞特性;在TMZ作用后,这类细胞比例增加,提示其与化疗耐药有关。Objective To explore the relationship between chemotherapy resistance and cancer stem cells. Methods The changes of the number and protein expression were analyzed before and after the CD133＋ cells in glioma U251 were intervented by temozolomide（TMZ）. The expressions of CD133,nestin,neuron-specific enolase（NSE）,glial fibrillary acidic protein（GFAP） and other proteins in glioma U251 cells were detected by immunofluorescence and Western blot. Results U251 cells in the TMZ group did not join The proportion of CD133＋ cells was significantly greater in the U251 cells with TMZ intervention than that without（0. 337±0. 012 vs. 0. 060±0. 003）（P〈0. 05）. After TMZ intervention,the proportion of nestin-positive cells in the CD133＋ cells were increased, but GFAP and NSE-positive cells were decreased（P〈0. 05）. Conclusion There CD133＋ cells in the U251 glioma cell lin＇es,majority of which have the properties of brain tumor stem cells. After TMZ intervention, an increased proportion of the CD133＋ cells suggests its relationship with chemotherapy-related drug resistance.

关 键 词：胶质瘤 替莫唑按 化疗耐药 CD133+细胞 

分 类 号：R739[医药卫生—肿瘤]