人脑胶质瘤PTEN及PI3K/AKT/GSK3相关蛋白的表达  

Expressions of PTEN and PI3K/AKT/GSK3 signaling transduction pathway-associated proteins in human glioma

作　　者：高岩[1] 鲁晓杰[1] 马涛[1] 王清[1] 

机构地区：[1]南京医科大学附属无锡二院神经外科,江苏省214002

出　　处：《江苏医药》2012年第10期1165-1167,共3页Jiangsu Medical Journal

摘　　要：目的探讨人脑胶质瘤及正常脑组织中PTEN及AKT、pAKT、GSK3、pGSK3的表达差异。方法将组织标本分为正常脑组织、低级别胶质瘤、高级别胶质瘤三组,采用Western blot方法检测PTEN、AKT、pAKT、GSK3、pGSK3的表达。结果 PTEN在正常脑组织中表达量最高,在低级别胶质瘤中表达量居中,在高级别胶质瘤中表达量最低(P<0.05)。pAKT及pGSK3则在高级别胶质瘤中表达量最高,低级别胶质瘤中表达量居中,正常脑组织中表达量最低(P<0.05)。AKT及GSK3在三种标本中表达量没有明显差别(P>0.05)。结论 PTEN、pAKT、pGSK3蛋白的表达水平与胶质瘤病理分级具有相关性。随着胶质瘤级别的增高,PTEN蛋白的表达量明显降低,pAKT、pGSK3的表达量明显增高。Objective To investigate the expressions of PTEN, pAKT, AKT,GSK3 and pGSK3 in different grades of glioma and human normal brain tissues. Methods Tissue samples were divided into three groups of A（low-grade glioma）, B（high-grade glioma） and C（normal brain tissue）. The expressions of PTEN, AKT, pAKT, GSK3, pGSK3 were detected by Western blot. Results The expression level of PTEN was in an order of group C〉group A〉group B （P〈0. 05）. The expression levels of pAKT and pGSK3 were in an order of group B 〉 group A 〉 group C （ P〈0. 05 ）. The expression levels of AKT and GSK3 were not significantly different among groups of A, B and C （P〉0. 05）. Conclusion The expression levels of PTEN, pAKT and pGSK3 are correlated with pathological grades of human glioma. The higher grade of glioma, the lower expression level of PTEN, and the higher expression levels of DAKT and pGSK3.

关 键 词：PTEN AKT GSK3 脑胶质瘤 

分 类 号：R602[医药卫生—外科学]