不同方法转染人前列腺癌PC-3细胞pEGFP-N1基因的体外实验研究  

Study of pEGFP-N1 transfection into human prostate cancer cell PC-3 by different transfecticion methods in vitro

作　　者：吴作辉[1,2] 白文坤[1] 张吉臻[1] 张跃力[1] 申锷[1] 胡兵[1] 

机构地区：[1]上海交通大学附属第六人民医院超声医学科,上海超声研究所,上海200233 [2]遵义医学院附属医院超声科

出　　处：《临床泌尿外科杂志》2012年第6期466-469,共4页Journal of Clinical Urology

基　　金：上海市科学技术委员会重点项目(编号:10JC1412600)

摘　　要：目的:探讨转染人前列腺癌PC-3细胞pEGFP-N1基因的最佳转染方法。方法:以超声微泡造影剂、超声辐照、脂质体转染及其相互结合的方法,将质粒pEGFP-N1基因转染人前列腺癌PC-3细胞,24h后以荧光显微镜观察前列腺癌PC-3细胞中的绿色荧光蛋白表达情况,并用流式细胞仪测定转染率。结果:以超声+微泡+脂质体组基因转染效率最高,与其他组比较,差异均有统计学意义(P<0.05)。结论:超声联合微泡与脂质体结合能明显提高pEGFP-N1基因在人前列腺癌细胞中的转染率,是一种较理想的基因转染方法。Objective： To find a better method to transfer pEGFP-N1 into human PC-3 prostate cancer cell. Methods： Ultrasound contrast agent microbubbles, ultrasound, and lipofection method or combined with each other were used to transfer plasmid pEGFP into human prostate cancer PC-3 cells. The expression of pEGFP-N1 was studied by fluorescepce microscope and flow cytometry 24 hours after transfection. Results： Ultrasound combined with mierobubble and liposome group had the best efficiency and had significant difference compared to other groups（P〈0. 05）. Conclusions： The use of ultrasound, in combination with microbubbles, could be a potential physical method for increasing liposome gene delivery efficiency.

关 键 词：前列腺癌细胞 超声 微泡 脂质体 基因转染 

分 类 号：R737.25[医药卫生—肿瘤] Q782.4[医药卫生—临床医学]