十种苯丙氨酸衍生物对小鼠黑色素瘤细胞系B16的作用研究  

The Effects of Ten Kinds of Phenylalanine Derivatives on Mouse Melanoma Cell Line B16

作　　者：陈冉[1,2] 闫春玲[1,2] 王彬彬[1,2] 闫文娟[1,2] 王书佩[1,2] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南省-科技部共建细胞分化调控国家重点实验室培育基地,新乡453007

出　　处：《中国细胞生物学学报》2012年第6期573-579,共7页Chinese Journal of Cell Biology

基　　金：国家973项目前期研究专项(No.2010CB534905)资助项目~~

摘　　要：该文研究了十种苯丙氨酸(Phe)的对位衍生物Fp、Clp、Bp、Ip、Ap、Np、Sp、Mp、Pp、Cp对小鼠黑色素瘤细胞系B16的细胞毒性、诱导细胞凋亡作用和抑制细胞成集落作用。结果表明,它们的细胞毒性大小依次为Pp、Fp、Mp、Ip、Sp、Ap、Bp。其中Mp和Ip的毒性相近,Sp和Ap的毒性相近。它们诱导细胞凋亡的作用强弱依次为Fp、Mp、Ip。其中Mp和Ip的作用相近。它们抑制细胞成集落的作用大小依次为Pp、Fp、Ip、Mp、Sp、Bp、Np、Ap。其中Pp、Fp、Ip、Mp的作用相近,Sp、Bp、Np的作用相近。初步的细胞毒理分析表明,Fp、Mp、Ip能够诱导B16细胞凋亡和抑制B16细胞形成集落。We studied the effects of 10 kinds of phenylalanine-bit derivatives on mouse melanoma cell line B16,which included cytotoxicity,induction of apoptosis and inhibition of cells into the colony.The 10 kinds of phenylalanine derivatives included Fp,Clp,Bp,Ip,Np,Sp,Pp,Ap,Mp,Cp.The results showed that the cytotoxicity of above phenylalanine derivatives to the B16 cell line was in sequence as follows： Pp,Fp,Mp,Ip,Sp,Ap,Bp.The cytotoxicity of Mp and Ip was similar,and the cytotoxicity of Sp and Bp was similar.The apoptosis-inducing activity for them were in sequence： Fp,Mp,Ip,while,the role of Mp and Ip was similar.The inhibition of cloney-forming unit arranged in a sequence： Pp,Fp,Ip,Mp,Sp,Bp,Np,Ap.The inhibition roles of Pp,Fp,Mp and Ip were similar,and that of Sp,Bp and Np were similar.Preliminarily cytotoxicity analysis showed that Fp,Mp,Ip induced B16 cell line apoptosis by cell nuclear and inhibited colony formation of cells.

关 键 词：苯丙氨酸衍生物 小鼠黑色素瘤细胞系B16 细胞毒性(IC50) 细胞凋亡 细胞成集落 

分 类 号：R739.5[医药卫生—肿瘤]