影响NOD/SCID小鼠淋巴瘤^(18)F-FDG PET摄取的免疫因素分析  

Analysis of immune factors on uptake of ^(18)F-FDG in lymphoma of NOD/SCID mice

作　　者：张苏蕾[1] 张建华[1] 王荣福[1] 闫平[1] 陈立新[1] 

机构地区：[1]北京大学第一医院核医学科,北京100034

出　　处：《中国医学影像技术》2012年第6期1027-1030,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家重点基础研究发展计划(973计划)项目(2006CB705705);国家自然科学基金(39870241;81071183;30270410)

摘　　要：目的通过比较两种不同类型免疫缺陷动物肿瘤组织的18F-FDG标准摄取值(SUV),初步探讨B、NK细胞免疫与肿瘤组织的相互作用。方法对NOD/SCID小鼠(T、B、NK免疫联合缺陷)12只,BALB/C裸鼠(无T细胞免疫)12只,分别种植淋巴瘤细胞(A20),成瘤后对各组均经尾静脉注射18 F-FDG 8.32～12.02 MBq(225～325μCi),使用Philips小动物PET进行全身动态和静态扫描,观察B细胞免疫和NK细胞自然杀伤免疫对肿瘤组织摄取18 F-FDG的影响,并分析免疫因素在肿瘤摄取18F-FDG中的作用。结果 NOD/SCID小鼠最大SUV(SUVmax)为18.33±3.42,高于BALB/C裸鼠[(9.66±4.15),t=4.981,P<0.01],平均SUV(SUVmean)为11.04±1.40,高于BALB/C裸鼠[(5.47±3.30),t=4.718,P=0.001]。结论不同免疫状态对小鼠淋巴瘤的18F-FDG摄取水平存在影响。Objective To explore the interactions between the B, NK cell-mediated immunity and tumor tissue by comparing the standardized uptake value （SUV） of ^18F-FDG uptake of tumor tissue with two different types of immune deficient mice. Methods Twelve NOD/SCID mice （T, B, NK combined immune deficiency） and 12 BALB/C nude mice （no T-cell immunity） were planted lymphoma cells （A20）. After the formation of the tumors, all mice were injected with ^18F-FDG 8.32-12.02 MBq （225-325μCi）. Whole body dynamic and static scan was performed on Philips Micro-PET to observe the impact of B cells and NK cells on the uptake of 18 F-FDG in lymphoma. And the role of immune factors in the tumor uptake of 18F-FDG was analyzed. Results NOD/SCID mice＇s maximal SUV （SUVmax） was higher （18.33±3.42） than BALB/C nude mice （9.66 ±4.15, t= 4. 981, P〈0.01）, while their mean SUV （SUV ） was also higher （11.04± 1.40） than that of nude mice （5.47±3.30, t=4. 718, P=0.01）. Conclusion Different immune statuses of mice affect the level of lymphoma uptake of 18 F-FDG.

关 键 词：淋巴瘤 正电子发射型体层摄影术 体层摄影术 X线计算机 18F氟脱氧葡萄糖 免疫学因素 

分 类 号：R-332[医药卫生] R817.4