非小细胞肺癌LVD、MVD、CEAmRNA及KAI1和Kiss-1的表达与患者预后关系的研究  

Relationship among the Expression of Lymphatic Vessel Density,Microvessel Density,Carcinoembryonic Antigenic mRNA,KAI1,and Kiss-1,and Prognosis in Patients with Non-small Cell Lung Cancer

作　　者：汪国文[1] 王祖义[1] 黎传奎[1] 王萍 柴大敏 承泽农 

机构地区：[1]蚌埠医学院第一附属医院胸心外科,安徽233004 [2]病理科

出　　处：《中国肺癌杂志》2012年第6期348-354,共7页Chinese Journal of Lung Cancer

基　　金：安徽省教育厅科研基金(No.2002KJ220,No.KJ2008B53ZC);蚌埠医学院科研基金(No.BY1026)资助~~

摘　　要：背景与目的目前对非小细胞肺癌(non-small cell lung cancer,NSCLC)患者预后的研究很多,有些临床病理因素已被公认与预后有关,本研究旨在对淋巴管密度(lymphatic vessel density,LVD)、微血管密度(microves-sel density,MVD)及癌胚抗原信使核糖核酸(carcinoembryonic antigen messenger ribonucleic acid,CEAmRNA)和肿瘤转移抑制基因KAI1、Kiss-1的表达与NSCLC患者预后的关系进行探讨。方法选取57例NSCLC患者,采用逆转录巢式聚合酶链反应(nested-RT-PCR)和微流控芯片(micro-fluid chip)技术对外周血CEAmRNA的表达进行检测;采用免疫组织化学技术对肺癌组织LVD、MVD和KAI1、Kiss-1的表达进行检测,并对所有患者进行随访分析。结果全组的5年生存率为18%,中位生存期为34个月;单因素分析显示TNM分期、淋巴结有无转移、CEAmRNA的阳性表达、MVD、LVD和Kiss-1的表达对生存有影响;多因素分析显示TNM分期、淋巴结有无转移和CEAmRNA的表达是影响预后的独立危险因素。结论在NSCLC患者中MVD、LVD和Kiss-1及CEAmRNA的表达可以在一定程度上反映患者的预后。Background and objective In recent years, many studies have revealed the prognosis in patients with non-small cell lung cancer （NSCLC）. In general, some clinic-pathological parameters have been related with prognosis. The aim of this study is to detect the relationship among lymphatic vessel density （LVD）, microvessel density （MVD）, expression of carcinoembryonic antigen （CEA） mRNA, metastasis suppressor genes （KAI1 and Kiss-1 ）, and the prognosis of NSCLC patients. Methods Blood samples were collected from 57 cases of NSCLC. The transcription of CEA mRNA was detected via nested reverse transcriptase-polymerase chain reaction and micro-fluid chip. Immunohistochemistry was performed to detect the expression of LVD, MVD, KAI1 and Kiss-1 in the patients. All follow-up data were collected and analyzed. Results The overall five-year survival rate was 18%, and the median survival was 34 months. TNM stage, lymph node metastasis, and ex- pression of MVD, LVD, CEA mRNA and Kiss-1 were factors to survival, as determined via single survival analysis. Multivariate analysis demonstrated that TNM stage, lymph node metastasis, and expression of CEA mRNA were independent prognostic factors for NSCLC patients. Conclusion The expression ofMVD, LVD, Kiss-1 and CEA mRNA is related to the prognosis of NSCLC.

关 键 词：肺肿瘤 癌胚抗原 肿瘤转移抑制基因 KAI1 KISS-1 预后 

分 类 号：R734.2[医药卫生—肿瘤]