女性散发性基底细胞型乳腺癌ERα启动子甲基化的研究  

Estrogen receptor-alpha promoter methylation in sporadic basal-like breast cancer

作　　者：毛晓韵[1] 房月[2] 井明晰[1] 范垂锋[3] 魏晶[1] 姚凡[1] 刘崇[1] 金锋[1] 

机构地区：[1]中国医科大学附属第一医院普通外科教研室,肿瘤外科,乳腺外科,辽宁沈阳110001 [2]中国医科大学药学院药理学教研室,辽宁沈阳110001 [3]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2012年第10期738-742,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30950009)

摘　　要：目的:探讨ERα甲基化与基底细胞型乳腺癌发生发展的相关性。方法:用甲基化PCR研究60例散发性基底型乳腺癌ERα启动子甲基化情况,并研究其与临床病因素之间的关系。结果:女性散发性基底细胞型乳腺癌组织中ERα基因启动子甲基化发生率为80.0%(48/60)。ERα基因启动子甲基化与淋巴结转移情况、肿瘤分期、p53蛋白、BRCA-1蛋白和BRCA-2蛋白表达情况有相关性,与患者年龄及绝经状态无关。结论:ERα基因启动子甲基化在基底细胞型乳腺癌发生发展中可能起重要作用。OBJECTIVE： To discuss the promoter methylation of estrogen receptor-alpha in sporadic basal-like breast cancer. METHODS： The methylation of ERa promoter was investigated in genomic DNA of 60 sporadic basal-like breast cancer by methylation-specific polymerase chain reaction. The correlation between ERα methylation and clinicopathological features of basal-like breast cancer was analyzed. RESULTS： ERα methylation was observed in 48 of 60 （80.0 %） sporadic basal-like breast cancer. No correlation was found between the ERα methylation and age,menopausal status,while it was significantly associated with lymph node metastasis,tumor stage,nuclear p53 accumulation,BRCA-1 and BRCA-2 expression in sporadic basal-like breast cancer. CONCLUSION： ERα methylation maybe plays an important role in basal-like breast carcinogenesiss.

关 键 词：乳腺肿瘤 受体 雌激素 甲基化 

分 类 号：R737.9[医药卫生—肿瘤]