含十字形结构域蛋白-3对肺癌细胞增殖与迁徙的影响研究  

Effect of Jumonji Domain-containing Protein-3 on the Proliferation and Migration of Lung Cancer Cell Line

作　　者：田翠杰[1] 邓宏宇[2] 汤小菊[3] 胡贺芳[1] 刘小菁[3] 罗凤鸣[2] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041 [2]四川大学华西医院金卡医院,成都610041 [3]四川大学华西医院心血管研究室Ⅱ/再生医学中心,成都610041

出　　处：《生物医学工程学杂志》2012年第3期514-518,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30500222;30971325;30871118);教育部新世纪优秀人才资助项目(NCET-08-0374);四川省科技厅杰出青年基金资助项目(09ZQ026-020;2009SZ0190)

摘　　要：为了解含十字形结构域蛋白-3(JMJD3)对肺癌细胞A549增殖与迁徙的影响,通过JMJD3表达质粒转染A549培养48h后,EDU染色及流式细胞术检测细胞增殖与凋亡,划痕实验检测细胞迁徙,RT-PCR检测p21mR-NA表达。结果显示,JMJD3转染后EDU阳性比例明显高于对照(JMJD3:40.75%±2.07%,对照:20.97%±1.5%,P<0.001);G1期细胞比例降低(JMJD3:47.80%±1.85%,对照:54.60%±0.95%,P=0.005)。转染JM-JD3后p21mRNA表达量为35.89%±3.71%,而对照为91.78%±3.74%,两组p21表达变化明显(P<0.001)。转染JMJD3质粒24h和48h后细胞迁徙距离分别为(0.47±0.27)cm和(0.96±0.40)cm,对照为(0.57±0.22)cm和(1.08±0.33)cm,两组无显著性差异(P>0.05)。JMJD3可促进A549细胞增殖,降低G1期细胞比例并下调p21mRNA表达,但对A549迁徙无明显影响。For investigating the effect of Jumonji domain-containing protein-3 （JMJD3） on the behavior of lung cancer cell line, A549 proliferation was measured with EDU staining and flow cytometer after JMJD3 expression plasmid and pcDNA3.1 transfection at 48h. The migration ability of A549 was tested at the same time. The expression of p21 mRNA was measured with RT-PCR. The results showed that JMJD3 transfection increased the EDU positive cells ratio （J MJD3：40.75% ± 2.07% ,control： 20.97 %± 1.5 %, P〈0. 001）. G1 phase cell ration also increased af- ter J MJ D3 transfeetion（J MJ D3 ： 47.80%± 1.85 %, control ： 54.60 %±0.95%, P = 0. 005 ）. The mRNA expression of p21 decreased inJMJD3 group （JMJD3： 35.89%±3.71%,control： 91.78%±3.74%, P〈0.001）. The distances of migration were （0. 47±0.27）cm and （0.96±0. 40）cm after 24h and 48h with JMJD3 tranfection, compared to （0.57±0.22）cm and （1.08±0.33）cm in control, respectively （P〉0.05）. JMJD3 promoted the prolifera tion of A549 and decreased the G1 cell numbers, decreased the p21 mRNA, but had no effect on A549 migration.

关 键 词：含十字形结构域蛋白-3 肺癌 细胞增殖 细胞迁徙 

分 类 号：R734.2[医药卫生—肿瘤]