甘蓝胞质雄性不育(OguCMS)相关的MYB转录因子BoMYB1的克隆与表达分析  

Cloning and Expression of an Ogura Cytoplasmic Male Sterile(OguCMS)-related MYB Transcription Factor in Brassica oleracea var.capitata

作　　者：张磊[1,2] 康宗利[1] 刘海霞[2] 康俊根[2] 

机构地区：[1]沈阳农业大学,沈阳110866 [2]北京市农林科学院蔬菜研究中心,北京100097

出　　处：《农业生物技术学报》2012年第6期627-635,共9页Journal of Agricultural Biotechnology

基　　金：“十二五”国家科技支撑计划项目(No.2012BAD02B01);国家自然科学基金(No.31171958);北京市自然科学基金项目(No.6102012)

摘　　要：萝卜胞质雄性不育(OguCMS)是目前甘蓝中应用较广的雄性不育类型,MYB转录因子具有调控植物防御应答反应和多个发育过程的作用。本实验以在甘蓝(Brassica oleracea var.capitata)OguCMS花药中下调10.3倍的EST序列为信息探针,结合电子克隆及RACE技术,得到一个与甘蓝OguCMS雄性不育相关的MYB转录因子全长cDNA,命名为BoMYB1(GenBank登录号:JN703995)。经亚细胞定位预测,该基因定位于细胞核,全长1141bp,包含一个长度为196bp的5'非翻译区、246bp的3'非翻译区和一个699bp的开放阅读框。该基因在花药中具表达优势,并在花药发育晚期出现表达高峰,受植物激素水杨酸(SA)和茉莉酸甲酯(JA-ME)的调控,诱导花药发育基因的表达。实验结果提示,BoMYB1可能是参与OguCMS花药发育的重要基因之一。Ogura cytoplasmic male sterile （OguCMS） is the most widely used male sterile type in cabbage breeding. MYB transcription factors play a key role in regulation of plant defense response and multiple development processes. In present experiment, a R2R3-MYB transcription factor which down regulated 10.3 times in cabbage （Brassica oleracea var. capitata） OguCMS lines was cloned by SMART RACE strategy.The fulllength cDNA of BOMYB1 was 1 141 bp, which contained a 196 bp long 5＇ untranslated region, a 246 bp long 3＇ untranslated region and a 699 bp long open reading frame （GenBank accession number： JN703995）. It was localized in the nucleus by subcellular localization prediction. It was an anther preferentially expressed gene in cabbage, which reached its expression peak in the late development. It was induced by the regulation ofplant hormones salicylic acid（SA） and jasmonate methyl （JA-ME）, and consequently regulated the expression of anther development genes. The experimental results suggests that BOMYB1 may be one of the important genes which involved ija OguC, MS anther development.

关 键 词：甘蓝 MYB转录因子 RACE技术 序列分析 激素应答 

分 类 号：S634.3[农业科学—蔬菜学]