鸡VLDLR基因多态性与蛋黄性状的关联分析  

Association of Polymorphisms of VLDLR Gene with Yolk Traits in Chicken

作　　者：曹顶国[1,2] 周艳[1,2] 雷秋霞[1,2] 韩海霞[1,2] 李福伟[1,2] 李桂明[1,2] 盛倩[1,3] 王存芳[3] 逯岩[1,2] 

机构地区：[1]山东省农业科学院家禽研究所,济南250023 [2]山东省家禽育种工程技术研究中心,济南250023 [3]山东轻工业学院食品与生物工程学院,济南250353

出　　处：《畜牧兽医学报》2012年第6期849-856,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省良种工程重大项目(2008lz015)

摘　　要：本研究采用PCR-RFLP方法,检测VLDLR基因在山东地方鸡种汶上芦花鸡、济宁百日鸡和莱芜黑鸡中的多态性,并分析各多态位点不同基因型与4个蛋黄性状的相关性。结果表明,在3个供试群体中共检测到2个具有生物学意义的SNPs位点,即第6外显子8 467nt处的G→A突变(V8467)和第17内含子13 876nt处的A→G突变(V13876)。最小二乘分析结果表明,V8467对蛋黄质量有极显著影响(P<0.01),对蛋黄高度有显著影响(P<0.05);V13876对蛋黄质量有极显著影响(P<0.01),对蛋黄比率和蛋黄高度有显著影响(P<0.05);而双倍型对蛋黄质量、蛋黄比率的影响均达到极显著水平(P<0.01),对蛋黄高度的影响达到显著水平(P<0.05)。结果提示:本研究检测到的VLDLR基因的V8467和V13876多态性位点可以作为蛋黄性状的候选分子标记。The SNPs of VLDLR gene in Wenshang Luhua chicken, Jining Bairi chicken and Laiwu Black chicken were detected by PCR-RFLP method. And the correlation between the polymor- phism loci and the yolk traits were analyzed. As a result, a G/A mutation at nt 8 467 in VLDLR- exon6 and an A/G mutation at nt 13 876 in VLDLR-intronl7 were identified. The least square analysis showed that the V8467 was significantly associated with yolk weight （P〈0.01） and yolk height （P〈0.05） ; the V13876 was significantly associated with yolk weight （P〈0.01）, yolk rate （P〈0.05） and yolk height （P〈0.05） ; the diplotype was significantly associated with yolk weight （P〈0.01）, yolk rate （P〈0.01） and yolk height （P〈0.05）. These results indicated that V8467 and V13876 loci of VLDLR gene would be useful candidate markers in selection program on yolk traits.

关 键 词：鸡 VLDLR基因 蛋黄性状 SNPS 单倍型 PCR—RFLP 

分 类 号：S831.2[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]