Purification of Astaxanthin from Laminaria japonica by Ionic Liquid-based Monolithic Cartridge  

作　　者：YOON Chang-hwan ROW Kyung-ho 

机构地区：[1]Department of Chemical Engineering,Inha University,Incheon 402-751,Korea

出　　处：《Chemical Research in Chinese Universities》2012年第3期406-409,共4页高等学校化学研究（英文版）

基　　金：Supported by the Korea Sea Grant Program(Gyeong-gi Sea Grant) Funded by the Ministry of Land,Transport and Maritime Affairs,Korea

摘　　要：An effective and accurate method was developed for the extraction of astaxanthin from Laminariajapo- nica with subsequent separation by ionic liquid-based monolithic cartridge. The optimized extraction conditions in- cluding extraction solvent（ethanol）, extraction time（90 min） and ultrasonic power（75 W） were obtained by systema- tical investigation. Chromatographic analysis was performed on a C18 column with ultraviolet（UV） detection at 476 nm, and a solution consisting of methanol/acetonitrile/H2O/dichloromethane（83：6：6：5, volume ratio） was used as the mobile phase at a flow rate of 0.7 mL/min. After ionic liquid-based monolithic solid phase extraction, 17.82 μg/g as- taxanthin was obtained from Laminaria japonica. This ionic liquid-based monolithic cartridge exhibits high affinity and selectivity for astaxanthin, and it can be potentially used as the stationary phase of high performance liquid chromatography（HPLC）.An effective and accurate method was developed for the extraction of astaxanthin from Laminariajapo- nica with subsequent separation by ionic liquid-based monolithic cartridge. The optimized extraction conditions in- cluding extraction solvent（ethanol）, extraction time（90 min） and ultrasonic power（75 W） were obtained by systema- tical investigation. Chromatographic analysis was performed on a C18 column with ultraviolet（UV） detection at 476 nm, and a solution consisting of methanol/acetonitrile/H2O/dichloromethane（83：6：6：5, volume ratio） was used as the mobile phase at a flow rate of 0.7 mL/min. After ionic liquid-based monolithic solid phase extraction, 17.82 μg/g as- taxanthin was obtained from Laminaria japonica. This ionic liquid-based monolithic cartridge exhibits high affinity and selectivity for astaxanthin, and it can be potentially used as the stationary phase of high performance liquid chromatography（HPLC）.

关 键 词：Laminariajaponica ASTAXANTHIN EXTRACTION Ionic liquid Cartridge 

分 类 号：TS201.2[轻工技术与工程—食品科学]