c-myc、c-myb基因在白血病骨髓基质细胞中的表达及相关性研究  

Expression of c-myc and c-myb Gene in Leukemic Bone Marrow Stromal Cells and Their Correlation

作　　者：张莹[1] 陈蕊[1] 张炜[1] 李娟[1] 陈轩[1] 赵丽[1] 

机构地区：[1]兰州大学第一医院中心实验室,甘肃兰州730000

出　　处：《中国实验血液学杂志》2012年第3期531-535,共5页Journal of Experimental Hematology

基　　金：甘肃省科技攻关项目(编号2GS042-A43-014-19);甘肃省科技厅科技支撑计划项目(编号1011FKCA129)

摘　　要：本研究检测分析c-myc、c-myb原癌基因在白血病细胞和骨髓基质细胞(BMSC)中的表达及其相关性。用逆转录聚合酶链反应(RT-PCR)技术定量分析原癌基因c-myc、c-myb的表达水平,通过流式细胞术分析白血病细胞和BMSC的膜表面抗原,同时采用染色体显带技术分析细胞核型。结果表明:①c-myc、c-myb基因在白血病细胞及其BMSC中均有一定的表达,尤其是在染色体异常的白血病细胞中高表达,其均值分别为1.15±0.38和1.03±0.48,与对照组比较均具有统计学差异(P<0.05);在染色体异常的BMSC中其均值分别为2.08±0.82(P<0.05)和1.46±0.29(P<0.05);②在白血病细胞和BMSC中,c-myc mRNA、c-myb mRNA的表达水平均与血小板计数具有相关性;③在不同预后组中,c-myc mRNA、c-myb mRNA表达呈线性相关;④在白血病细胞及急性组BMSC中原癌基因c-myc、c-myb的表达存在正相关;⑤白血病细胞中c-myc的表达量分别与BMSC中c-myc、c-myb的表达量有相关性。结论:在白血病中降低c-myc或c-myb原癌基因的表达水平可能成为治疗白血病的一种治疗方案。This study was purposed to investigate the expression level of oncogene c-myc and c-myb in leukemic cells and leukemic bone marrow stromal cells（BMSC） and the correlation with each other.The expression levels of c-myc and c-myb in those cells were examined semi-quantitatively by reverse transcription polymerase chain reaction（RT-PCR）.Flow cytometry analysis was used to detect the membrane surface antigens of leukemic cells and BMSC.The karyotype was analyzed by G-banding techniques.The results showed that（1）c-myc and c-myb gene were expressed in the normal control group,the leukemic cells and BMSC of patients group.The mean expression levels of c-myb mRNA and c-myc mRNA in abnormal chromosomal leukemic cells were 1.03±0.48 and 1.15±0.38 respectively,which were significantly higher than those in control group（P〈0.05）.In the abnormal karyotype stromal cells,the mean expression level of c-myb mRNA and c-myc mRNA were 2.08±0.82 and 1.46±0.29 respectively（P〈0.05）.（2） The expression level of c-myc and c-myb mRNA were closely associated with patients′ platelet counts（P〈0.05）.（3） The expression of c-myc mRNA linearly correlated with the expression of c-myb mRNA in different prognostic groups.（4） In acute leukemic cells and BMSC,c-myc expression positively correlated with c-myb expression.（5） The expression level of c-myc in leukemic cells correlated with the expression levels of c-myc and c-myb in BMSC,respectively.It is concluded that the reduction of c-myc or c-myb expression levels may be a therapeutic regimen for leukemia.

关 键 词：C-MYC C-MYB 白血病 骨髓基质细胞 

分 类 号：R733.7[医药卫生—肿瘤]