氯法拉滨对NB4细胞的增殖抑制作用及对Bcl-2表达的影响  

Effect of Clofarabine on Proliferation and Bcl-2 Expression of NB4 Cells

作　　者：刘海波[1] 张梅[1] 李呈亮[1] 贺鹏程[1] 

机构地区：[1]西安交通大学医学院第一附属医院血液内科,陕西西安710061

出　　处：《中国实验血液学杂志》2012年第3期571-573,共3页Journal of Experimental Hematology

摘　　要：本研究观察氯法拉滨对人急性髓系白血病NB4细胞的增殖抑制作用并探讨其可能的作用机制。用MTT法观察氯法拉滨0.01-0.1μmol/L作用于NB4细胞48 h的增殖抑制作用;氯法拉滨0.01-0.1μmol/L作用于NB4细胞24 h后,用流式细胞术分析其细胞的凋亡水平,Western blot检测细胞内Bcl-2的蛋白表达。结果表明,氯法拉滨对NB4细胞具有浓度依赖性增殖抑制作用。氯法拉滨作用24 h后,NB4细胞凋亡率明显增加,Bcl-2蛋白表达下调。结论:氯法拉滨能抑制NB4细胞增殖,其作用机制可能与下调Bcl-2的蛋白表达,诱导NB4细胞凋亡有关。The aim of this study was to observe the effect of clofarabine on proliferation of NB4 cells and its possible mechanism.MTT method was used to detect proliferation of NB4 cells treated with clofarabine 0.01-0.1 μmol/L for 48 h.The treated with clofarabine 0.01-0.1 μmol/L for 24 h,apoptosis rate and Bcl-2 expression of NB4 cells were measured by flow cytometry and Western blot respectively.The results showed that clofarabine inhibited proliferation of NB4 cells in a concentration-depended manner（r=0.78）.After treated with clofarabine for 24 h,apoptosis rate of NB4 cells increased and Bcl-2 expression in NB4 cells decreased obviously（P〈0.05）.It is concluded that clofarabine inhibits proliferation of NB4 cells,which may be related with the down-regulation of Bcl-2 and induction of apoptosis.

关 键 词：氯法拉滨 NB4细胞 BCL-2表达 

分 类 号：R733.71[医药卫生—肿瘤] R979.31[医药卫生—临床医学]