1,25(OH)_2维生素D_3对人树突状细胞成熟及其介导免疫耐受的影响  

Influence of 1,25(OH)_2 Vitamin D_3 on Maturation of Human Dendritic Cells and DC-mediated Immune Tolerance

作　　者：彭静[1] 曹祥山[1] 邱国强[1] 孙冠星[1] 

机构地区：[1]苏州大学附属第三医院常州市第一人民医院血液科,江苏常州213003

出　　处：《中国实验血液学杂志》2012年第3期736-739,共4页Journal of Experimental Hematology

基　　金：常州市卫生局科研项目(编号201001)

摘　　要：本研究旨在探讨1,25(OH)2维生素D3〔1,25(OH)2Vit D3〕对人树突状细胞(DC)分化、成熟及功能的影响及其机制。在体外将人外周血单个核细胞诱导分化成DC,实验组加入1,25(OH)2Vit D31 nmol/L培养9 d,对照组加入等量无水乙醇,流式细胞仪检测DC表面共刺激因子表达水平。混合淋巴细胞培养后,用MTT法评估DC刺激同种异体T细胞增殖的能力。Western blot检测DC吲哚胺2,3-双加氧酶(IDO)蛋白表达。结果表明,与对照组相比,实验组DC表面标志CD80、CD83、CD86表达率低于对照组(P<0.05),CD1a高于对照组(P<0.05);CD80、CD83、CD86、CD1a表达率分别为(40.43±9.83)%、(20.04±4.73)%、(14.45±5.38)%,(58.48±10.72)%;对照组CD80、CD83、CD86、CD1a表达率分别为(29.36±13.34)%、(35.91±10.19)%、(27.15±11.64)、(72.20±12.79)%。实验组DC刺激同种异体T细胞增殖的能力受到抑制;DC表达IDO蛋白上调。结论:1,25(OH)2Vit D3抑制DC的成熟,通过上调DC的IDO蛋白表达抑制DC刺激同种异体T淋巴细胞增殖及介导免疫耐受。This study was aimed to investigate the effect of 1,25（OH）2 vitamin D3（1,25（OH）2 Vit D3） on the differentiation,maturation and function of human dendritic cells（DC） in vitro and its mechanism.Human peripheral blood mononuclear cells were induced to differentiate to DC in vitro.The DC in test group were cultured with 1,25（OH）2 Vit D3 1 nmol/L for 9 d,whlie the DC in control group were cultured with the equivalent of absolute alcohol.The expression of co-stimulatory molecules on DC were analyzed by flow cytometry.T cell proliferation induced by DC was assessed by MTT method.The expression of inoleamine 2,3-dioxygenase（IDO） protein was determined by Western blot.The results showed that compared with the control group,the expression of CD80,CD83 and CD86 on DC in test group was significantly down-regulated（P〈0.05）,while the CD1a was up-regulated（P〈0.05）.The expression rate of CD80,CD83,CD86,CD1a in test group were（40.43±9.83）%,（20.04±4.73）%,（14.45±5.38）%,（58.48±10.72）% respectively,while in control group were（29.36±13.34）%,（35.91±10.19） %,（27.15±11.64）%,（72.20±12.79）% respectively.Compared with the control group,1,25（OH）2 Vit D3-treated DC exhibited a markedly reduced ability to stimulate allogenic T cell proliferation and up-regulated IDO protein expression.It is concluded that 1,25（OH）2 Vit D3 efficiently inhibits the maturation of DC and DC-mediated T cell proliferation,which may be related to the up-regulation of IDO protein expression.

关 键 词：1 25(OH)2D3 树突状细胞 吲哚胺2 3-双加氧酶 免疫耐受 

分 类 号：R979.5[医药卫生—药品]