尾静脉注射K562细胞建立慢性髓系白血病小鼠模型及其鉴定  

Establishment and Identification of CML Model via Injection of K562 cells into the Murine Caudal Vein

作　　者：张佳[1] 杨文华[1] 杨向东[1] 史哲新[1] 王兴丽[1] 于文俊[1] 郝征[1] 

机构地区：[1]天津中医药大学第一附属医院血液科,天津300193

出　　处：《中国实验血液学杂志》2012年第3期773-776,共4页Journal of Experimental Hematology

基　　金：天津市高等学校博士学科点专项科研基金(编号20091210110004)

摘　　要：本研究通过给NOD/SCID小鼠尾静脉注射K562细胞的方式,建立人慢性髓系白血病(CML)-NOD/SCID小鼠髓外浸润模型,并通过组织病理学检查及RT-PCR检测BCR-ABL融合基因等进行模型鉴定。将24只NOD/SCID小鼠经137Cs全身照射,剂量270 cGy,吸收剂量率80 cGy/min,之后随机分为实验Ⅰ组、实验Ⅱ组和对照组,每组8只。照射后24 h内,实验组小鼠经尾静脉注射K562细胞,实验Ⅰ组:5×106/只,实验Ⅱ组:1×107/只;对照组注射生理盐水0.2 ml/只。持续观察小鼠的一般情况和生存时间,应用组织病理学检查和RT-PCR方法检测白血病细胞髓外浸润表现。结果表明,注射4-8周后,2个实验组均发生白血病细胞髓外浸润,提示成功建立CML/NOD-SCID小鼠髓外浸润模型。结论:应用尾静脉注射K562细胞的方法可成功建立CML/NOD-SCID小鼠髓外浸润模型。通过组织病理学检查及RT-PCR检测BCR-ABL融合基因可对模型进行鉴定。This study was purposed to establish and identify the model of extramedullary infiltration of CML-NOD/SCID mice.24 mice were irradiated with 270 cGy of 137Cs and absorbed dose rate 80 cGy/min,and were randomly divided into test group Ⅰ,test groupⅡ and control group.The mice in test group Ⅰ and test group Ⅱ were injected with 5×106 and 1×107 K562 cells per mouse respectively,the mice in control group were injected with 0.2 ml of normal saline.The general situation and survival time of these mice were monitored,the extramedullary infiltration of leukemia cells was detected by histopathology examination and RT-PCR.The results indicated that at 4-8 weeks after injection,all the mice of group Ⅰand groupⅡ displayed extramedullary infiltration,suggesting that CML/NOD-SCID model was successfully established.It is concluded that the model of extramedullary infiltration of CML/NOD-SCID mice can be established by injection K562 cells into caudal vein,and can be comfirmed by histopathologic examination and detection of BCR-ABL fusion gene using RT-PCR.

关 键 词：NOD/SCID小鼠 髓外浸润 白血病动物模型 

分 类 号：R733.72[医药卫生—肿瘤] R332[医药卫生—临床医学]