芪苈强心对心肌梗死后大鼠心肌细胞凋亡的影响  

Effect of Qiliqiangxin on cardiomyocyte apoptosis in rats after myocardial infarction

作　　者：肖骏[1] 马渝[1] 邓松柏[2] 佘强[2] 黄开顺[3] 

机构地区：[1]重庆市急救医疗中心心内科,重庆400014 [2]重庆医科大学第二附属医院心内科,重庆400010 [3]重庆医科大学基础研究所,重庆400016

出　　处：《中国病理生理杂志》2012年第6期1045-1050,共6页Chinese Journal of Pathophysiology

摘　　要：目的:探讨芪苈强心对心肌梗死大鼠心肌细胞凋亡的影响。方法:结扎冠脉前降支制作大鼠心肌梗死模型,随机分为假手术组(sham,n=5)、心肌梗死组(MI,n=16)和芪苈强心组(4 g.kg-1.d-1,n=15),28d后检测心肌梗死面积,TUNEL法检测心肌细胞凋亡指数(AI),免疫组化法检测Fas蛋白表达,Western blotting检测黄嘌呤氧化酶(XO)和caspase-3蛋白表达,比色法测定XO和清除活性氧活性。结果:MI组非梗死区心肌细胞AI升高,Fas、XO和caspase-3蛋白表达增强,XO活性增加,清除活性氧活性降低(P<0.01)。芪苈强心组与MI组比较,非梗死区心肌细胞AI降低,Fas和caspase-3蛋白表达减少,XO活性降低,清除活性氧活性升高(P<0.01),但梗死面积和XO蛋白表达在两组间无显著差异(P>0.05)。结论:芪苈强心能够抑制非梗死区心肌细胞凋亡,其作用机制可能与减少活性氧簇生成及降低Fas和caspase-3的表达有关。AIM： To explore the effect of traditional Chinese medicine Qiliqiangxin on cardiomyocyte apoptosis after myocardial infarction （MI） in a rat model. METHODS： MI was induced in rats by ligation of the anterior descending coronary artery. The survivors were randomly divided into 3 groups： sham operation group, MI group and Qiliqiangxin treatment group （4 g穔g-1穌-1）. After 28 days, the infarction size was measured. Apoptotic index （AI） was determined by TUNEL. The expression of Fas was detected by the method of immunohistochemistry in non-infarcted zones （NIZ）, and the expression of xanthine oxidase （XO） and caspase-3 in myocardial tissue from NIZ was detected by Western blotting. In addition, XO activity, and O2 -？ and OH？ scavenging activity of myocardial tissues in the NIZ were measured by colorimetry. RESULTS： Compared with MI group, AI and the expression of Fas and caspase-3 in the NIZ were significantly depressed in Qiliqiangxin treatment group. Moreover, the activity of XO was significantly decreased while O2 -？ and OH穝cavenging activity was significantly increased in Qiliqiangxin treatment group. Ventricular remodeling was attenuated. No significant difference in infarct size and XO expression level between Qiliqiangxin treatment group and MI group was observed. CONCLUSION： Qiliqiangxin may inhibit apoptosis of cardiomyocytes in the NIZ of rats by reducing reactive oxygen species and depressing the expression of Fas and caspase-3.

关 键 词：芪苈强心 黄嘌呤氧化酶 活性氧簇 细胞凋亡 心肌梗死 

分 类 号：R542.2[医药卫生—心血管疾病]