乙肝病毒X蛋白相互作用蛋白促进HepG2细胞迁移并调节β-catenin表达  

Overexpression of HBXIP in HepG2 cells induces cell migration and regulates expression of β-catenin

作　　者：崔利园[1] 张钊瑞[1] 费洪荣[2] 姚树桐[3] 李晓倩[1] 王凤泽[1] 

机构地区：[1]泰山医学院生物科学学院,山东泰安271016 [2]泰山医学院药学院,山东泰安271016 [3]泰山医学院基础医学院,山东泰安271016

出　　处：《中国病理生理杂志》2012年第6期1128-1131,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30800422);泰山医学院博士基金资助项目

摘　　要：目的:检测乙型肝炎病毒X蛋白相互作用蛋白(hepatitis B virus X-interacting protein,HBXIP)对HepG2肝癌细胞迁移及β-catenin表达的影响,为深入研究HBXIP与肝细胞癌发生发展的相关性奠定基础。方法:以建立的稳定高表达HBXIP的HepG2细胞系为实验材料,Transwell实验观察HBXIP高表达对细胞迁移能力的影响;明胶酶谱分析法检测基质金属蛋白酶9(MMP-9)的活性;免疫印迹实验检测MMP-9、糖原合成酶激酶3β(GSK-3β)、p-GSK-3β、β-catenin及p-β-catenin的表达。结果:Transwell实验结果表明,HBXIP能够促进HepG2细胞的迁移能力;高表达HBXIP的肝癌细胞中,MMP-9的活性及蛋白表达水平均升高;免疫印迹结果表明HBXIP促进了β-catenin的表达,并抑制β-catenin的磷酸化;同时促进GSK-3β(Ser9)的磷酸化。结论:HBXIP与肿瘤细胞的迁移密切相关,HBXIP高表达促进肝癌细胞的迁移可能是其调控GSK-3β/β-catenin表达的结果。AIM： To investigate the effects of hepatitis B virus X-interacting protein（HBXIP） in hepatic cancer cells on the cell migration and expression of β-catenin. METHODS： Transwell assay was used to assess the cell migration. Gelatin zymography was used to observe the activity of matrix metalloproteinase 9 （MMP-9）. The expression of MMP-9, glycogen synthase kinase 3β（GSK-3β）, p-GSK3β, β-catenin and p-β-catenin in HepG2 cells was determined by Western blotting. RESULTS： HepG2 cells which stably overexpressed HBXIP （HepG2-HBXIP） exhibited higher migration ability than the control cells. The results of the gelatin zymography assay showed that HBXIP overexpression increased the activity of MMP-9 in HepG2 cells. The results of Western blotting indicated that HBXIP increased the expression of MMP-9 and β-catenin, inhibited the phosphorylation of β-catenin and promoted the phosphorylation of GSK-3β （Ser9）. CONCLUSION： HBXIP regulates the GSK-3β/β-catenin signaling pathway, resulting in a significant improvement of hepatocellular carcinoma cell migration.

关 键 词：肝肿瘤 乙型肝炎病毒X蛋白相互作用蛋白 基质金属蛋白酶9 Β-连环蛋白 

分 类 号：R733[医药卫生—肿瘤] R34[医药卫生—临床医学]