针刺对创伤性脑损伤大鼠脑组织EGF和bFGF表达的影响  

Effect of acupuncture on expression of EGF and bFGF in brain tissues of rats with traumatic brain injury

作　　者：张毅敏[1] 唐纯志[2] 程少冰[1] 陈爱连[1] 张玉卿[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632 [2]广州中医药大学针灸推拿学院,广东广州510010

出　　处：《中国病理生理杂志》2012年第6期1132-1134,1139,共4页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.9151063201000028);广州市科技支撑计划资助项目(No.2010GN-E00221)

摘　　要：目的:观察针刺对创伤性脑损伤模型大鼠脑组织表皮生长因子(EGF)和碱性成纤维生长因子(bFGF)表达的影响。方法:参照Feeney自由落体冲击造模法建立创伤性脑损伤大鼠模型,将30只大鼠随机分为假手术组、模型组和针刺组,每组10只。针刺组给予针刺治疗,每天1次,共治疗7 d。处理结束后,采用免疫组织化学方法检测损伤脑组织EGF和bFGF含量。结果:与假手术组比较,模型组大鼠脑组织EGF表达下降(P<0.01),而bFGF上升(P<0.01);与模型组比较,针刺组EGF和bFGF表达明显升高(P<0.01)。结论:针刺可促进神经再生相关生长因子EGF和bFGF的表达,这可能是针刺促进神经再生和功能恢复、治疗颅脑损伤的机制之一。AIM： To observe the effect of acupuncture on the expression of epidermal growth factor （EGF） and basic fibrolblast growth factor （bFGF） in the brain tissues of rats with traumatic brain injury. METHODS： Thirty SD rats were randomized into sham-operated group, model group and acupuncture group. The model of traumatic brain injury was established by free drop impact. Acupuncture was performed to the rats in acupuncture group once every day and 7 days altogether. Brain histotomy was conducted after the treatment. Immunohistochemical method was adopted to test the protein expression of EGF and bFGF. RESULTS： Compared to sham-operated group, the expression of EGF in the brain tissues of model group decreased （P〈0.01）, and the expression of bFGF increased （P〈0.01）. Compared to model group, the expression of EGF and bFGF in acupuncture group increased obviously （P〈0.01）. CONCLUSION： Acupuncture significantly increases the expression of EGF and bFGF, and improves the repair of injured brain tissues. This might be one of the mechanisms by which acupuncture can treat traumatic brain injury and improve the nervous function.

关 键 词：创伤性脑损伤 针刺 表皮生长因子 碱性成纤维生长因子 大鼠 

分 类 号：R363[医药卫生—病理学]